Structural and biophysical studies of synaptic vesicle fusion machinery
Abstract/Contents
- Abstract
- A defining feature of eukaryotic cells is the compartmentalization of membrane-bound organelles with specialized functions. Movement of cargo between compartments, and exchange of material into and out of a cell are fundamental to cellular viability and effective communication with other cells. Secretion of neurotransmitters, the process by which neurons relay information, occurs when neurotransmitter-containing vesicles fuse with the target membrane in a pre-synaptic nerve terminal. Members of the SNARE, SM, and AAA+ ATPase protein families are central mediators of the exquisitely regulated process of neurotransmission. SNARE proteins form a complex that drives the fusion of synaptic vesicles with the target membrane; the SM protein Munc18a is a crucial mediator of SNARE complex assembly, and the ATPase NSF disassembles SNARE complex following neurotransmitter secretion, recycling SNAREs for subsequent rounds of vesicle fusion. The work presented here describes structural, biophysical, and biochemical approaches used to investigate the mechanism by which Munc18a, SNAREs and NSF orchestrate synaptic vesicle fusion.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2013 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Colbert, Karen Newton |
|---|---|
| Associated with | Stanford University, Department of Structural Biology. |
| Primary advisor | Weis, William I |
| Thesis advisor | Weis, William I |
| Thesis advisor | Brünger, Axel T |
| Thesis advisor | Garner, Craig |
| Advisor | Brünger, Axel T |
| Advisor | Garner, Craig |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Karen Newton Colbert. |
|---|---|
| Note | Submitted to the Department of Structural Biology. |
| Thesis | Ph.D. Stanford University 2013 |
| Location | electronic resource |
Access conditions
- Copyright
- © 2013 by Karen Newton Colbert
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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