Engineered biomaterials for therapeutic optimization of cardiovascular interventions
Abstract/Contents
- Abstract
- Ischemic heart disease continues to represent the leading cause of death worldwide, annually accounting for 10 million deaths globally and 1 in 7 deaths in the United States. Current treatment of severe coronary disease and acute myocardial infarction typically relies on reperfusion therapies, including fibrinolytic therapy, percutaneous coronary intervention, and coronary artery bypass grafting. These methods, while successful at restoring macrovascular blood flow after infarction, inadequately address residual microvascular perfusion deficits resulting in cardiomyocyte death, downstream ventricular remodeling, progression to heart failure, and early mortality. Additionally, following myocardial infarction and/or a cardiac operation, patients can develop Dressler's syndrome, or post-cardiac injury syndrome, where inflammation and scarring form cardiac adhesions resulting in increased risks during secondary cardiac operations. This dissertation discusses several projects related to ischemic heart disease and its multiple sequelae. This work demonstrates improved human mesenchymal stem cell (hMSC) culture techniques by culturing hMSCs on chemically oxidized polystyrene substrates. This technology could be utilized in cell therapy applications where timely access to high quantities of hMSCs is difficult due to cell culture constraints. This thesis also examines the use of a photosynthetic oxygen delivery system that rescues the myocardium following acute ischemia. Finally, the use of a dynamically crosslinked polymer-nanoparticle (PNP) hydrogel adhesion barrier is explored for the prevention of cardiac post-operative adhesions or post-cardiac injury syndrome. The PNP hydrogel adhesion barrier significantly reduced adhesions in both rodent and ovine models of cardiac adhesions. These results are expanded to post-operative peritoneal adhesions and result in a significant reduction in peritoneal adhesions following abdominal surgery
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2020; ©2020 |
| Publication date | 2020; 2020 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Stapleton, Lyndsay M |
|---|---|
| Degree supervisor | Woo, Joseph |
| Thesis advisor | Woo, Joseph |
| Thesis advisor | Appel, Eric (Eric Andrew) |
| Thesis advisor | Cochran, Jennifer R |
| Degree committee member | Appel, Eric (Eric Andrew) |
| Degree committee member | Cochran, Jennifer R |
| Associated with | Stanford University, Department of Bioengineering |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Lyndsay Mariah Stapleton |
|---|---|
| Note | Submitted to the Department of Bioengineering |
| Thesis | Thesis Ph.D. Stanford University 2020 |
| Location | electronic resource |
Access conditions
- Copyright
- © 2020 by Lyndsay M Stapleton
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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