Microfabricated hydrogel platforms to compare single cells in 2D vs 3D engineered microenvironments
Abstract/Contents
- Abstract
- I developed novel platforms to investigate the effects of 2D vs 3D controlled microenvironments on cells. Cells can sense and adapt to their local mechanical environment. In the body, cells exist in 3D environments, highly structured for the cell's given purpose. I wanted to investigate the effects of 3D microenvironments on single cells in a very controlled manner, thus allowing comparisons between 2D and 3D. The motivation of this work is to eventually study heart muscle cells, or cardiomyocytes, though the platforms I developed could be applied to numerous biological models. In this thesis, I provide an introduction to cardiomyocytes (Chapter 1) and engineered 2D and 3D microenvironments that have been developed by the field (Chapter 2). I then introduce my platforms to constrain cells to 2D or 3D microenvironments and provide characterization of the geometry and stiffness (Chapter 3). I next show simple mechanical models that provide insight into the mechanical loading environment for cells in each of the platforms (Chapter 4). Next, I show that 3D microenvironments can drastically affect cell cytoskeletal structure and nuclear shape. I also describe the development of a different platform, which enables patterning two proteins on 3D platforms using only soft lithography techniques (Chapter 6). Last, I provide conclusions and future directions for the work, providing ideas for how my controlled 2D and 3D microenvironments could be implemented to gain more understanding of single cells (Chapter 7).
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2019; ©2019 |
| Publication date | 2019; 2019 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Wilson, Robin Elizabeth |
|---|---|
| Degree supervisor | Chaudhuri, Ovijit |
| Degree supervisor | Pruitt, Beth |
| Thesis advisor | Chaudhuri, Ovijit |
| Thesis advisor | Pruitt, Beth |
| Thesis advisor | Bernstein, Daniel, 1953- |
| Degree committee member | Bernstein, Daniel, 1953- |
| Associated with | Stanford University, Department of Mechanical Engineering. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Robin Wilson. |
|---|---|
| Note | Submitted to the Department of Mechanical Engineering. |
| Thesis | Thesis Ph.D. Stanford University 2019. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2019 by Robin Elizabeth Wilson
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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