Single-walled carbon nanotubes (SWNTs) as near infrared fluorescent imaging agents in biological systems
Abstract/Contents
- Abstract
- The near infrared range has long been known to be advantageous for biological imaging and sensing applications. In particular, the second near infrared window (1000 nm -- 1400 nm, NIR II) is characterized by low endogenous autofluorescence and deep tissue penetration due to reduced tissue scatter. To address the dearth of fluorophores in this window, this work focuses on utilizing the intrinsic near infrared photoluminescence of semiconducting single walled carbon nanotubes (SWNTs) to take advantage of this unique spectral region. First, SWNTs are made bio-inert and applied as fluorescent probes for highly specific cellular targeting using antibodies such as Rituxan and Herceptin. These probes were then used for the first whole animal fluorescent imaging using SWNTs in vivo following tail vein injection, including the observation of high SWNT accumulation in tumors. By implementing a surfactant exchange method to improve the fluorescence yield of SWNTs solubilized by phospholipid-polyethylene glycol, high magnification intravital microscopy of tumor vessels beneath thick skin was achieved. Further improvements were made to improve the fluorescence yield of the SWNT probes by utilizing a density gradient separation method which removed poorly fluorescent short tubes and nanotube bundles. Another separation method, ion-exchange chromatography was applied to isolate single chirality SWNTs and perform multicolor NIR imaging in vitro and in vivo. Finally, these bright, biocompatible nanotube fluorophores were used to achieve video rate imaging of mice in vivo during tail vein injection for dynamic contrast enhanced imaging through principal component analysis. The emission in the NIR II region allowed crisp anatomical resolution, confirmed by mock tissue phantom studies and Monte Carlo simulation.
Description
| Alternative title | Single-walled carbon nanotubes as near infrared fluorescent imaging agents in biological systems |
|---|---|
| Type of resource | text |
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2010 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Welsher, Kevin David |
|---|---|
| Associated with | Stanford University, Department of Chemistry |
| Primary advisor | Dai, Hongjie, 1966- |
| Thesis advisor | Dai, Hongjie, 1966- |
| Thesis advisor | Chidsey, Christopher E. D. (Christopher Elisha Dunn) |
| Thesis advisor | Zare, Richard N |
| Advisor | Chidsey, Christopher E. D. (Christopher Elisha Dunn) |
| Advisor | Zare, Richard N |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Kevin David Welsher. |
|---|---|
| Note | Submitted to the Department of Chemistry. |
| Thesis | Ph.D. Stanford University 2010 |
| Location | electronic resource |
Access conditions
- Copyright
- © 2010 by Kevin David Welsher
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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