A new model for the structure and composition of the inversin compartment
Abstract/Contents
- Abstract
- Primary cilia in many cell types contain a peri-axonemal sub-compartment called the inversin compartment. Four proteins have been found to assemble within the inversin compartment: INVS, ANKS6, NEK8, and NPHP3. The function of the inversin compartment is unknown but appears to be critical for normal development including left-right asymmetry and renal tissue homeostasis. Here we combine super-resolution imaging of human RPE1 cells, a classic model for studying primary cilia in vitro, with a genetic dissection of the protein-protein relationships important for compartment assembly to develop a new structural model. We propose that INVS is the core structural determinant of a compartment composed of novel fibril-like substructures which we identify here by 3D single-molecule super-resolution imaging. We find that NEK8 and ANKS6 depend on INVS for localization to the compartment, but NEK8-ANKS6 density within the compartment is intrinsically regulated by NEK8. Together NEK8 and ANKS6 are required downstream of INVS to localize and concentrate NPHP3 within the compartment. These results provide a more detailed structure for the inversin compartment and introduce a new example of a membrane-less compartment organized by protein-protein interactions
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2019; ©2019 |
| Publication date | 2019; 2019 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Bennett, Henrietta Wulsin |
|---|---|
| Degree supervisor | Jackson, Peter K. (Peter Kent) |
| Thesis advisor | Jackson, Peter K. (Peter Kent) |
| Thesis advisor | Cimprich, Karlene |
| Thesis advisor | Graves, Edward (Edward Elliot), 1974- |
| Thesis advisor | Meyer, Tobias |
| Degree committee member | Cimprich, Karlene |
| Degree committee member | Graves, Edward (Edward Elliot), 1974- |
| Degree committee member | Meyer, Tobias |
| Associated with | Stanford University, Cancer Biology Program. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Henrietta Bennett |
|---|---|
| Note | Submitted to the Cancer Biology Program |
| Thesis | Thesis Ph.D. Stanford University 2019 |
| Location | electronic resource |
Access conditions
- Copyright
- © 2019 by Henrietta Wulsin Bennett
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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