3D Printing of Bioengineered Functional Cardiac Tissues

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Abstract/Contents

Abstract: Heart disease is the world’s leading cause of mortality, accounting for over 7.4 million deaths each year. Consequently, there has been immense interest in the medical and scientific community to develop technologies to help regenerate injured hearts. 3D printing of biological materials (i.e., 3D bioprinting) is an innovative technology that may be applied to help us understand and treat cardiac disease. In my thesis work, I utilized 3D bioprinting to create a viable and functional artificial cardiac construct. Human umbilical vein endothelial cells (HUVECs) and human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) were encapsulated in 10% gelatin methacrylate (GelMA) hydrogels and subsequently bioprinted into 3D tissue constructs. Analysis of the printed tissues over time demonstrated cell viability as assessed by their metabolic activity in culture. Cell migration and proliferation were observed in the printed HUVEC tissues 7 days post printing and beating was observed in the hiPSC-CM tissues 3 days post printing. In addition, I demonstrated the endothelialization of acellular printed scaffolds using a perfusion bioreactor system. In the future, I plan to utilize the 3D tissue printing and bioreactor platform for in vitro applications such as personalized drug cardiotoxicity screening and in vivo applications such as generating a cardiac patch for transplantation into an animal model of myocardial infarction.

Type of resource	text
Date created	May 3, 2017

Author	Hu, Daniel
Principal investigator	Wu, Sean
Degree granting institution	Stanford University, Department of Bioengineering
Contributing author	Serpooshan, Vahid
Contributing author	Hu, James
Contributing author	Venkatraman, Sneha

Subject	tissue engineering
Subject	cardiovascular medicine
Subject	stem cell biology
Subject	3D bioprinting
Subject	3D printing
Subject	biomaterials
Subject	cardiac tissue engineering
Subject	Stanford Cardiovascular Institute
Subject	Stanford Department of Bioengineering
Genre	Thesis

Location	https://purl.stanford.edu/zg634zz0920

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License: This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).

Preferred Citation: Hu, Daniel; Serpooshan, Vahid; Hu, James; enkatraman, Sneha; and Wu, Sean . (2017). 3D Printing of Bioengineered Functional Cardiac Tissues. Stanford Digital Repository. Available at: http://purl.stanford.edu/zg634zz0920

Undergraduate Theses, School of Engineering

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