Regulation of gene expression by RNA-binding proteins and microRNAs
Abstract/Contents
- Abstract
- Circumstantial evidence suggests vast post-transcriptional regulatory networks in eukaryotes. There are scores of examples of mRNAs with rich post-transcriptional lives, and dozens of RNA-binding proteins and noncoding RNAs are suggested to have regulatory functions. While eukaryotic genomes encode hundreds of RNA-binding proteins and metazoans also encode dozens to hundreds of regulatory noncoding RNAs, few RNA-binding proteins or regulatory noncoding RNAs have been studied systematically, leaving unanswered basic questions about the scope and organization of post-transcriptional regulation in any eukaryote. First, I present studies aimed at uncovering some of the basic principles of post-transcriptional regulation in eukaryotes, via searching for the RNA targets of 40 proteins in the yeast Saccharomyces cerevisiae: a selective sample of the approximately 600 annotated and predicted RBPs as well as several proteins not annotated as RBPs. Second, I present a simple biochemical approach to identify direct targets of specific microRNAs, important noncoding regulatory RNAs, that regulate protein synthesis of most mammalian mRNAs. Third, I present experiments that uncover the magnitude and respective contributions of translational repression and mRNA decay of microRNA-mediated regulation. These studies apply simple, yet powerful approaches to systematically dissect various aspects of post-transcriptional regulation and uncover a wealth of novel information regarding regulation by RNA-binding proteins and microRNAs.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Copyright date | 2010 |
| Publication date | 2009, c2010; 2009 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Hogan, Daniel Joseph |
|---|---|
| Associated with | Stanford University, Department of Biochemistry. |
| Primary advisor | Brown, Patrick O'Reilly, 1954- |
| Primary advisor | Herschlag, Daniel |
| Thesis advisor | Brown, Patrick O'Reilly, 1954- |
| Thesis advisor | Herschlag, Daniel |
| Thesis advisor | Ferrell, James Ellsworth |
| Thesis advisor | Stearns, Tim |
| Advisor | Ferrell, James Ellsworth |
| Advisor | Stearns, Tim |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Daniel Joseph Hogan. |
|---|---|
| Note | Submitted to the Department of Biochemistry. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2010. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2010 by Daniel Joseph Hogan
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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