Plasmonic nanomaterials for biological sensing and biomedical diagnostics
Abstract/Contents
- Abstract
- This thesis describes the development of plasmonic nanomaterials and their spectroscopic applications in biological sensing and clinical diagnostic applications. Gold nanomaterials and their assemblies possess unique localized surface plasmon resonances in the visible and near-infrared (NIR). The enhancement of both the local electric field and NIR fluorescence emission rate via the coupling of scattering nanoparticle plasmon modes can lead to NIR fluorescence enhancement (NIR-FE) under appropriate conditions. Here, those conditions are optimized to yield the NIR-FE effect in the context of immunoassays. A novel solution-phase methodology was developed to prepare plasmonic chip and beads at large scale. Surface chemistry were explored on the so-called "nano-bio interface" for improved biocompatibility, enhanced targeted biomolecule immobilization, and reduced non-specific binding. Multiplexed protein assays are constructed on the nanostructured, plasmonic gold film to enjoy ~ 100-fold fluorescence enhancement, extending the protein/nucleic acid detection dynamic range by ~3 orders of magnitude towards the 1fM regime. Compared to conventional assays on commercial substrates, the plasmonic surface based immunoassays achieve vastly higher signal-to-noise ratios and broader dynamic range, owing to the large fluorescence boost and unique surface chemistry. The methodology developed affords high sensitivity detection of cancer biomarkers, cytokines, antibodies, DNA and microRNA, offering a broad dynamic range applicable to a wide variety of assays. The fast, simple, sensitive, multiplex and low-cost immunoassay based on plasmonic nanomaterials has great potential in both research and clinical settings.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2016 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Zhang, Bo |
|---|---|
| Associated with | Stanford University, Department of Chemistry. |
| Primary advisor | Dai, Hongjie, 1966- |
| Thesis advisor | Dai, Hongjie, 1966- |
| Thesis advisor | Cui, Bianxiao |
| Thesis advisor | Utz, Paul |
| Advisor | Cui, Bianxiao |
| Advisor | Utz, Paul |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Bo Zhang. |
|---|---|
| Note | Submitted to the Department of Chemistry. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2016. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2016 by Bo Zhang
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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