Identification of a Myogenic and Highly Transplantable Tert Expressing Non-Satellite Stem Cell Population
Abstract/Contents
- Abstract
- Telomerase Reverse Transcriptase (Tert), a component of the telomerase holoenzyme complex, has been previously identified as a marker of stem cell populations across tissues. However, its role in skeletal muscle is poorly understood. In skeletal muscle, volumetric muscle loss injuries pose a unique challenge for cellular-based therapies, which so far have been relatively unsuccessful in preclinical models. Using a Tert-lineage tracing genetic mouse model, we identified a set of rare Tert expressing cells distributed throughout muscle. These Tert-lineage cells expand upon injury and contribute to myofiber regeneration in a delayed fashion. In addition, muscular regeneration upon repeated injuries is impaired by the loss of Tert in Tert expressing cells and their progeny. While a very small population of satellite cells express Tert, we also identified a population of Tert Expressing Multipotent Myogenic Progenitors (TEMMPros). These TEMMPros are multipotent and myogenic in vitro and via transplantation in a Tert-dependent manner. Furthermore, transplantation of these TEMMPros resulted in improved regeneration compared to satellite cells in a mouse model of volumetric muscle loss. Single-cell sequencing reveals that TEMMPros are transcriptionally distinct from other known skeletal muscle cell populations including satellite cells. These observations identify a novel subset of myogenic, non-satellite Tert expressing cells in mice that possess remarkable capacity for regeneration and may serve as a cell-based therapeutic for skeletal muscle damage.
Description
| Type of resource | text |
|---|---|
| Date modified | December 5, 2022 |
| Publication date | May 5, 2022; May 2022 |
Creators/Contributors
| Author | Kim, Stewart |
|---|---|
| Thesis advisor | Artandi, Steven |
| Thesis advisor | Morrison, Ashby |
| Thesis advisor | Mudgett, Mary Beth |
| Degree granting institution | Stanford University, Department of Biology |
Subjects
| Subject | Biology |
|---|---|
| Subject | Skeletal Muscle |
| Subject | Stem Cells |
| Subject | Telomerase |
| Subject | Regenerative Medicine |
| Genre | Text |
| Genre | Thesis |
Bibliographic information
Access conditions
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 4.0 International license (CC BY-NC).
Preferred citation
- Preferred citation
- Kim, S. and Artandi, S. (2023). Identification of a Myogenic and Highly Transplantable Tert Expressing Non-Satellite Stem Cell Population. Stanford Digital Repository. Available at https://purl.stanford.edu/ys097rr2365
Collection
Undergraduate Theses, Department of Biology, 2021-2022
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- Contact
- skim321@stanford.edu
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