Roles of brassinosteroids in regulating arabidopsis root development
Abstract/Contents
- Abstract
- The balance between stem cell maintenance and differentiation is crucial for optimal growth of plant roots, yet the factors controlling this balance are unknown. The work presented in this dissertation demonstrates that the spatial distribution of BR activity and its antagonistic interaction with auxin are crucial for dynamics of stem cell activity and root growth. Microscopic analysis reveals a spatiotemporal pattern of the BR-activated transcription factor BZR1 along the root developmental gradient. A low BZR1 activity in the stem cell niche is required for stem cell maintenance, while a high BZR1 activity in the transition-elongation zone is required for normal cell elongation. This BZR1 gradient is established by BR catabolism but is also modulated by graded auxin distribution in the root tip. Transcriptomic and genetic analyses indicate that BR and auxin act antagonistically in regulating developmental zone-specific transcriptomes and cell elongation, and that a BR-auxin balance is required for optimal root growth. Finally, BR transport and its roles in cell-cell communication were characterized by analyses of BZR1 subcellular localization and growth phenotypes in transgenic lines expressing BR biosynthesis genes in certain cells or tissues. The results suggest that endogenous BR undergoes short-distance transport in plants.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2014 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Sae-Seaw, Juthamas |
|---|---|
| Associated with | Stanford University, Department of Biology. |
| Primary advisor | Bergmann, Dominique |
| Primary advisor | Wang, Zhi-Yong, Dr |
| Thesis advisor | Bergmann, Dominique |
| Thesis advisor | Wang, Zhi-Yong, Dr |
| Thesis advisor | Long, Sharon |
| Advisor | Long, Sharon |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Juthamas Sae-Seaw. |
|---|---|
| Note | Submitted to the Department of Biology. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2014. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2014 by Juthamas Sae-Seaw
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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