Physical modeling of particle and chromosome dynamics in living cells
Abstract/Contents
- Abstract
- The cellular cytoplasm is a complex, viscoelastic medium whose properties result in a rich spectrum of transport behaviors for the biomolecules moving within it. Disrupting the organization of this medium can lead to a wide variety of disease states or even the death of the cell, thus a fundamental physical understanding of these effects could guide novel strategies for the treatment of infection and disease. Our work develops physical models to better predict the consequences of the intracellular medium on biomolecular motion and interpret the results from experimental particle tracking data of RNA-protein particles and chromosome loci in living cells. We employ Brownian dynamics simulations to investigate the effects of macromolecular crowding and finite sample sizes on the observed particle trajectories. We find that RNA-protein particles inside cells exhibit non-Gaussian behavior in addition to subdiffusive dynamics. We also show that relatively simple dynamic polymer models can describe the motion of chromosomal DNA inside bacterial cells during segregation and use this same framework to predict the dynamic correlations between two connected loci.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2016 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Lampo, Thomas |
|---|---|
| Associated with | Stanford University, Department of Chemical Engineering. |
| Primary advisor | Spakowitz, Andrew James |
| Thesis advisor | Spakowitz, Andrew James |
| Thesis advisor | Dunn, Alexander Robert |
| Thesis advisor | Straight, Aaron, 1966- |
| Advisor | Dunn, Alexander Robert |
| Advisor | Straight, Aaron, 1966- |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Thomas Lampo. |
|---|---|
| Note | Submitted to the Department of Chemical Engineering. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2016. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2016 by Thomas Jared Lampo
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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