Systems approach to unravel lymphoid development in human bone marrow
Abstract/Contents
- Abstract
- Hematopoietic stem and progenitor cells (HSPCs) in the bone marrow (BM) are the source of life-long replenish of immune cells in the humans. Among multiple lineages, T cell, B cell, NK cell, and Innate lymphoid cell (ILC) are classified as lymphoid cells, based on their morphology and developmental origin. However, mainly due to the limited experimental manipulations for human hematopoiesis studies, lymphopoiesis in human bone marrow still remains elusive. To overcome the experimental limitations, we leveraged the systems approach to analyze healthy human BM HSPCs. We conducted a highly multiplexed single-cell proteomic screen to construct a comprehensive protein expression map of human BM HSPCs. From there, we identified several lymphoid progenitor populations by their lymphoid specific functional protein markers. The target populations were assessed for their epigenetic priming for lymphoid lineages by chromatin accessibility. Furthermore, we utilized the protein expression information to identify surface markers to enrich for the target population, prospectively isolated, and functionally validated their lymphoid potentials ex vivo. This multi-omic study exemplifies the integration of a high-dimensional single-cell study with experimental validations. Moreover, we provide a fine-mapped human lymphopoiesis landscape in human hematopoiesis.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2023; ©2023 |
| Publication date | 2023; 2023 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Kim, Yeeun |
|---|---|
| Degree supervisor | Bendall, Sean |
| Degree supervisor | Greenleaf, William |
| Thesis advisor | Bendall, Sean |
| Thesis advisor | Greenleaf, William |
| Thesis advisor | Davis, Mark |
| Thesis advisor | Majeti, Ravindra |
| Degree committee member | Davis, Mark |
| Degree committee member | Majeti, Ravindra |
| Associated with | Stanford University, School of Medicine |
| Associated with | Stanford University, Department of Microbiology and Immunology |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Yeeun Kim. |
|---|---|
| Note | Submitted to the Department of Microbiology and Immunology. |
| Thesis | Thesis Ph.D. Stanford University 2023. |
| Location | https://purl.stanford.edu/yn086nh4480 |
Access conditions
- Copyright
- © 2023 by Yeeun Kim
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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