A cluster of neuropeptide S neurons regulates breathing and arousal
Abstract/Contents
- Abstract
- Neuropeptide S (NPS) is a highly conserved peptide found in all tetrapods that functions in the brain to promote heightened arousal; however, the subpopulations mediating these phenomena remain unknown. We generated mice expressing Cre recombinase from the Nps gene locus (NpsCre) and examined populations of NPS+ neurons in the lateral parabrachial area (LPBA), the peri-locus coeruleus (peri-LC) region of the pons, and the dorsomedial thalamus (DMT). We performed brain-wide mapping of input and output regions of NPS+ clusters and characterized expression patterns of the NPS receptor (NPSR1). While the activity of all three NPS+ subpopulations tracked with vigilance state, only NPS+ neurons of the LPBA exhibited both increased activity prior to wakefulness and decreased activity during REM sleep, similar to the behavioral phenotype observed upon NPSR1 activation. Accordingly, we found that activation of LPBA, but not peri-LC NPS+ neurons increased wake and reduced REM sleep. Furthermore, given the extended role of the LPBA in respiration, and the link between behavioral arousal and breathing rate, we demonstrated that LPBA-, but not peri-LC-NPS+ neuronal activation increased respiratory rate. Together, our data suggests that NPS+ neurons of the LPBA represent an unexplored subpopulation regulating breathing and they are sufficient to recapitulate the sleep/wake phenotypes observed with broad NPS system activation.
Description
| Type of resource | Dataset, text |
|---|---|
| Date created | [ca. November 2023] |
| Date modified | November 29, 2023; November 30, 2023; November 30, 2023 |
| Publication date | November 29, 2023 |
Creators/Contributors
| Author | Angelakos, Christopher |
https://orcid.org/0000-0003-1124-9138
(unverified)
|
|---|---|---|
| Author | de Lecea, Luis |
https://orcid.org/0000-0002-8921-5942
(unverified)
|
Subjects
| Subject | Neuropeptide S |
|---|---|
| Subject | Neuropeptide S Receptor |
| Subject | Sleep |
| Subject | Arousal (Physiology) |
| Subject | Fiber Photometry |
| Subject | Lateral Parabrachial |
| Subject | Breathing |
| Subject | Respiration |
| Subject | Neuropeptide circuits |
| Genre | Data |
| Genre | Database |
| Genre | Manuscript |
| Genre | Data sets |
| Genre | Dataset |
| Genre | Databases |
Bibliographic information
Access conditions
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 4.0 International license (CC BY-NC).
Preferred citation
- Preferred citation
- Angelakos, C. and de Lecea, L. (2023). A cluster of neuropeptide S neurons regulates breathing and arousal. Stanford Digital Repository. Available at https://purl.stanford.edu/yf106cj5998. https://doi.org/10.25740/yf106cj5998.
Collection
Stanford Research Data
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