Sequence-encoded structure and function, from RNA to chromatin
Abstract/Contents
- Abstract
- Our cells rely on information encoded in genetic material to carry out the diverse func- tions that maintain life. Decoding genomic information requires a deep understanding of cis-effects—i.e. how sequence encodes the structure and function of DNA, RNA, and proteins—but also the effects of trans-factors that enact the transfer of informa- tion between these biological macromolecules and regulate what genomic information becomes active at what time. This work addresses both cis and trans contributions to biological function. First, this thesis will present a transcription factor that causes widespread chromatin changes during Small Cell Lung Cancer progression, revealing an unexpected global reprogramming during metastasis. Second, this thesis will exam- ine cis effects on the energetics of RNA tertiary folding, relying on quantitative measure- ments of tertiary assembly energetics of more than 300,000 structured RNA variants. These data allowed dissection of how primary sequence and secondary structure affect the the conformational behavior of RNA elements. Together, these projects demon- strate the value of quantitative yet genomic-scale approaches to dissect the diverse ways biological functions can be encoded in genomic information.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2017 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Denny, Sarah Knight |
|---|---|
| Associated with | Stanford University, Biophysics Program. |
| Primary advisor | Greenleaf, William James |
| Thesis advisor | Greenleaf, William James |
| Thesis advisor | Das, Rhiju |
| Thesis advisor | Herschlag, Daniel |
| Thesis advisor | Straight, Aaron, 1966- |
| Advisor | Das, Rhiju |
| Advisor | Herschlag, Daniel |
| Advisor | Straight, Aaron, 1966- |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Sarah Knight Denny. |
|---|---|
| Note | Submitted to the Program in Biophysics. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2017. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2017 by Sarah Knight Denny
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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