Roles of AtACINUS and AtPNN in alternative splicing and developmental transitions in Arabidopsis
Abstract/Contents
- Abstract
- Seed germination and flowering are two major developmental transitions in the life cycle of plants; their precise control is crucial for species survival in nature and involves complex regulatory mechanisms. Here, we report that the Arabidopsis AtACINUS and AtPNN proteins redundantly play key roles in regulating seed germination and flowering through alternative splicing (AS) and transcriptional regulation of target genes, respectively. The acinus pnn double mutant lacking both AtACINUS and AtPNN, which share sequence similarity only within a protein-protein interaction domain, showed severe growth defects including dwarfism, delayed seed germination and late flowering, while neither acinus nor pnn single mutant showed obvious morphological defect. Transcriptomic analysis of acinus pnn showed high levels of the flowering repressor FLC and RNAs responsive to abscisic acid (ABA), the hormone required for seed dormancy. The acinus pnn mutant also showed intron retention in many transcripts, including those encoding two negative regulators of ABA signaling: ABA-HYPERSENSITIVE 1 (ABH1) and HYPERSENSITIVE TO ABA 1 (HAB1). Consistent with these alterations of ABA-related transcripts, the acinus pnn mutant was hypersensitive to ABA treatment. Proteomic analyses indicate that AtACINUS is associated with the spliceosome and a large number of RNA-binding/processing proteins. AtACINUS associates with the RNAs of ABH1 and HAB1 and the promoter DNA of FLC in vivo, supporting its direct involvement in regulating AS and transcription. Our study uncovers important roles of AtACINUS/AtPNN in controlling key developmental transitions through dual functions in transcription and AS of target genes in Arabidopsis.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2018; ©2018 |
| Publication date | 2018; 2018 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Bi, Yang |
|---|---|
| Degree supervisor | Bergmann, Dominique |
| Degree supervisor | Wang, Zhi-Yong, Dr |
| Thesis advisor | Bergmann, Dominique |
| Thesis advisor | Wang, Zhi-Yong, Dr |
| Thesis advisor | Gozani, Or Pinchas |
| Thesis advisor | Mudgett, Mary Beth, 1967- |
| Degree committee member | Gozani, Or Pinchas |
| Degree committee member | Mudgett, Mary Beth, 1967- |
| Associated with | Stanford University, Department of Biology. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Yang Bi. |
|---|---|
| Note | Submitted to the Department of Biology. |
| Thesis | Thesis Ph.D. Stanford University 2018. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2018 by Yang Bi
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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