Engineering virus-like particles for targeted delivery of macromolecular therapeutics
Abstract/Contents
- Abstract
- Nanoparticles, carrying therapeutic cargo, offer a potential solution to a number of diseases or disorders where targeted delivery could improve efficacy while at the same time reducing off-target side-effects. Previously in the Swartz lab, virus-like particles (VLPs) from the Hepatitis B core viral capsid have been heavily engineered for stability and host immune system avoidance. Using this as a basis, I have expanded the functionalities of these VLPs to include improved VLP assembly, targeted cell specificity, and loading of therapeutic payloads. We have shown efficient loading of multiple types of cargo, including mimics of siRNA and therapeutic proteins, using VLPs that can assemble at significantly reduced ionic strengths. Using Prostate Cancer cells as a model disease target, we observed specificity of our construct for the targeted cells. Furthermore, these engineered VLPs can be easily modified for treating other cancers or genetic disorders and, in the future, for neurological disorders. The modularity and flexibility of this targeted delivery platform offers great promise for a broad range of medical applications.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2018; ©2018 |
| Publication date | 2018; 2018 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Rohovie, Marcus John |
|---|---|
| Degree supervisor | Swartz, James R |
| Thesis advisor | Swartz, James R |
| Thesis advisor | Covert, Markus |
| Thesis advisor | Khosla, Chaitan, 1964- |
| Degree committee member | Covert, Markus |
| Degree committee member | Khosla, Chaitan, 1964- |
| Associated with | Stanford University, Department of Chemical Engineering. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Marcus John Rohovie. |
|---|---|
| Note | Submitted to the Department of Chemical Engineering. |
| Thesis | Thesis Ph.D. Stanford University 2018. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2018 by Marcus John Rohovie
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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