Detection and quantitative characterization of biomolecular interactions with microfluidic mechanical trapping
Abstract/Contents
- Abstract
- In the era of rapid high-throughput DNA sequencing and increasing bioinformatic capabilities to analyze large amounts of biological data, it is especially important to continue to develop high-throughput experimental proteomic methods. Probing physical interactions among proteins and nucleic acids is a powerful approach to gain insight into their functional relationships. Microfluidic tools are of great potential value to this field because they make it possible to run hundreds or thousands of independent experiments in parallel and at a high spatial density. Here I present and explain MITOMI (mechanically induced trapping of molecular interactions), an in vitro method that was initially created to measure the affinities of transcription factors binding to DNA. I contributed to the development of the system so it could be applied to mapping protein-protein interaction networks, and used it to study the interactions of E. coli RNA polymerase in order to better understand the regulation of bacterial transcription. I also extended the utility of MITOMI by adapting it to be able to measure interaction kinetics and to more efficiently measure affinities. With the ability to discover interactions at a large scale and to quantitatively characterize them on the same platform, MITOMI constitutes a valuable contribution to proteomic methodology.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Copyright date | 2011 |
| Publication date | 2010, c2011; 2010 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Bates, Steven |
|---|---|
| Associated with | Stanford University, Department of Applied Physics |
| Primary advisor | Quake, Stephen Ronald |
| Thesis advisor | Quake, Stephen Ronald |
| Thesis advisor | Covert, Markus |
| Thesis advisor | Ferrell, James Ellsworth |
| Advisor | Covert, Markus |
| Advisor | Ferrell, James Ellsworth |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Steven R. Bates. |
|---|---|
| Note | Submitted to the Department of Applied Physics. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2011. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2011 by Steven Bates
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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