Biological imaging in a long-wavelength near-infrared window using novel nanomaterials
Abstract/Contents
- Abstract
- Fluorescence imaging in the second near-infrared window (NIR-II, 1000-1700 nm) has attracted increasing attention mainly owing to the advantage of reduced light scattering compared to imaging in shorter wavelength windows such as visible or first near-infrared window below 900 nm. The work in this thesis aims to develop novel nanomaterials with improved optical properties (e.g. high quantum yield and long emission wavelengths) and apply these nanomaterials for biological imaging in live animals, especially imaging in a long wavelength near-infrared window beyond 1500 nm (1500-1700 nm, named NIR-IIb window). Firstly, chirality-pure SWNTs with confined excitation and emission wavelengths were separated from the as-made SWNT mixture using a simple gel filtration method. Due to the strong resonance with the 808-nm excitation widely used for near-infrared imaging, the chirality-pure SWNTs showed much higher brightness compared to the pristine SWNT mixture, which significantly reduced the toxicity concern of using SWNTs for in vivo applications. Secondly, biological imaging in the 1500-1700 nm (NIR-IIb window), long end of the entire NIR-II window, was achieved using a novel large diameter SWNTs grown by laser vaporization method. NIR-IIb imaging is shown to be more advantageous than the traditional NIR-II window, owing to both reduced light scattering and reduced tissue autofluorescence. Thirdly, a bright, NIR-IIb emitting fluorophore, lead sulfide (PbS) quantum dots, was developed for real-time, deep-tissue imaging in the long wavelength NIR-IIb window. Due to the bright emission from the PbS quantum dots in the NIR-IIb region, real-time heart/lung imaging with high frame-rate of ~30 frames/second were performed with high resolution, revealing blood flow and cardiac cycle information useful for imaging heart and lungs.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2016 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Diao, Shuo |
|---|---|
| Associated with | Stanford University, Department of Chemistry. |
| Primary advisor | Dai, Hongjie, 1966- |
| Thesis advisor | Dai, Hongjie, 1966- |
| Thesis advisor | Cui, Bianxiao |
| Thesis advisor | Zare, Richard N |
| Advisor | Cui, Bianxiao |
| Advisor | Zare, Richard N |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Shuo Diao. |
|---|---|
| Note | Submitted to the Department of Chemistry. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2016. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2016 by Shuo Diao
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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