Investigation on diagnostic biomarkers and protein-protein interactions by using giant magnetoresistive biosensors
Abstract/Contents
- Abstract
- In the past decade, our research group has been developing giant magnetoresistive (GMR) biosensors and demonstrated that this sensor platform has great potential for application to many fields of nanomedicine, especially diagnostics and drug development. In this dissertation, I present major development and several new applications of GMR biosensors. First of all, the history of magnetic biosensors and GMR biosensors are introduced. Then, a new technique to longitudinally monitor immune response via antibody isotypes is described. This technique is used, in conjunction with bioluminescent imaging, to measure both firefly luciferase (FL) specific and total antibody response, and to find the upregulated IgG2a response in the regression mice. Cytokine measurement shows interferon-gamma signature in the regression mice. These results indicate that the immune response is predominantly involved with T-helper-1-cell-mediated immune response. Secondly, the use of protein and peptide microarrays for autoantibody detection is demonstrated. System lupus erythematosus is an autoimmune disease, characterized by the existence of autoantibodies. A chemokine score was previously proposed to assess disease severity. The correlation between autoantibodies measured by the microarray and the chemokine score is found, and the reactivity of autoantibodies to histone 2B peptides in chemokine high patients is higher than in chemokine low patients. Thirdly, a new kinetic assay using GMR biosensors is presented. The current gold standard, Surface Plasmon Resonance (SPR) is sensitive to changes in pH and salt concentration and is typically single-plex in nature. The GMR biosensor platform is insensitive to pH and salinity, and has an added ability to multiplex. By using magnetic nanoparticles conjugated with proteins and microfluidic chips, the interactions between programmed cell death 1 (PD-1) protein and its ligands are measured utilizing bivalent interactions and a Langmuir isotherm binding model. Lastly, the use of GMR biosensors as point-of-care (POC) devices is demonstrated in a Eigen Lifescience platform.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2015 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Lee, Jung-Rok |
|---|---|
| Associated with | Stanford University, Department of Mechanical Engineering. |
| Primary advisor | Santiago, Juan G |
| Primary advisor | Wang, Shan |
| Thesis advisor | Santiago, Juan G |
| Thesis advisor | Wang, Shan |
| Thesis advisor | Mallick, Parag, 1976- |
| Advisor | Mallick, Parag, 1976- |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Jung-Rok Lee. |
|---|---|
| Note | Submitted to the Department of Mechanical Engineering. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2015. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2015 by Jung-Rok Lee
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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