E3.03 Chaplin 2019 ReNUWIt Annual Meeting Poster

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Abstract/Contents

Abstract: Direct potable reuse (DPR), the direct reuse of advanced treated wastewater for drinking water, is a promising option for water-stressed cities, but methods to monitor DPR systems are needed to assess microbial quality throughout advanced treatment and distribution. Fluorescence-based flow cytometry is an emerging monitoring method for drinking water systems as it can be used to rapidly and inexpensively measure bacterial counts in water. However, there is minimal experience using flow cytometry to assess samples with low cell counts, such as samples taken after reverse osmosis treatment. The effect of experimental conditions (e.g. tube and run volumes) on Evian water was tested, and an increase in tube volume was found to increase measured cell numbers, while an increase in run volume decreased variation. Variations in flow cytometric results within the standard method suggest the importance of using consistent tube volumes and run volumes for samples of varying cell counts. In addition, blanks were created to mimic cell numbers found in advanced treated wastewater. Ultimately, this research will lead to guidelines for the use of flow cytometry at very low cell counts, including recommendations for experimental conditions that will minimize experimental noise and determining a quantification limit for intact and total cell count. These data can be used to better interpret the microbial water quality data of DPR systems.

Type of resource	other
Date created	May 2019

Subject	Re-inventing the Nation’s Urban Water Infrastructure
Subject	ReNUWIt
Subject	E3.03
Subject	Efficient Engineered Systems
Subject	Direct potable reuse
Subject	California

Location	https://purl.stanford.edu/wv128gv4181

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License: This work is licensed under an Open Data Commons Attribution License v1.0.

Preferred Citation: Chaplin, M., Miller, S.E., and Nelson, K.L. (2019). E3.03 Chaplin 2019 ReNUWIt Annual Meeting Poster. Stanford Digital Repository. Available at: https://purl.stanford.edu/wv128gv4181

Re-inventing the Nation's Urban Water Infrastructure (ReNUWIt)

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