Universal properties of the intrinsic microbial response to temperature fluctuations
Abstract/Contents
- Abstract
- The impact of temperature on growth is typically considered only under heat- or cold-shock conditions that elicit specific regulation. Over intermediate temperatures, the growth rate of all cells varies according to the Arrhenius law of thermodynamics; growth rate dynamics during transitions between temperatures remain mostly unstudied. Using a device that enables single-cell tracking during switches across a wide range of temperatures (0 °C to 47 °C), we show that many bacteria respond to temperatures upshifts on a characteristic time scale of ~1.6 doublings at the higher temperature, regardless of initial/final temperature or nutrient source. We rule out transcriptional, translational, and membrane reconfiguration as potential mechanisms, and instead discover that an autocatalytic enzyme network incorporating temperature-sensitive Michaelis-Menten kinetics recapitulates all temperature-shift dynamics and successfully predicts the altered temperature responses observed under simple-sugar and low-nutrient growth conditions. These findings suggest that the temperature sensitivity of metabolite flux dictates responses to temperature fluctuations.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2023; ©2023 |
| Publication date | 2023; 2023 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Knapp, Benjamin (Benjamin D.) |
|---|---|
| Degree supervisor | Huang, Kerwyn Casey, 1979- |
| Thesis advisor | Huang, Kerwyn Casey, 1979- |
| Thesis advisor | Cremer, Jonas |
| Thesis advisor | Sattely, Elizabeth |
| Degree committee member | Cremer, Jonas |
| Degree committee member | Sattely, Elizabeth |
| Associated with | Stanford University, School of Humanities and Sciences |
| Associated with | Stanford University, Biophysics Program |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Benjamin Knapp. |
|---|---|
| Note | Submitted to the Biophysics Program. |
| Thesis | Thesis Ph.D. Stanford University 2023. |
| Location | https://purl.stanford.edu/wq159cw3762 |
Access conditions
- Copyright
- © 2023 by Benjamin Knapp
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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