Development and application of a miniature, integrated fluorescence microscope for in vivo brain imaging
Abstract/Contents
- Abstract
- A longstanding goal in neuroscience is to understand animal behavior in terms of underlying cellular activity. Traditionally, conventional microscopes have not been suitable for imaging studies in awake behaving animals due to their large size and physical constraints. To overcome these limitations, we have developed a miniature (< 2 g mass), integrated fluorescence microscope to enable high-speed cellular imaging (20-100 Hz) in the brain of freely behaving mice. By combining the integrated microscope with an established mouse preparation for in vivo fluorescence imaging, we monitored calcium dynamics in many neurons simultaneously in mice performing typical behaviors such as running, grooming, and exploring an environment. The use of a recently developed genetically encoded calcium indicator, GCaMP3, permitted repeated observations of calcium dynamics in the same individual neurons at multiple times over the period of weeks to months. When additionally combined with image registration routines that track cellular identity across different imaging sessions, our system enables cellular-level monitoring of changes in calcium dynamics in a population of neurons over many weeks.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2012 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Burns, Laurie Davenport |
|---|---|
| Associated with | Stanford University, Department of Applied Physics |
| Primary advisor | Schnitzer, Mark Jacob, 1970- |
| Thesis advisor | Schnitzer, Mark Jacob, 1970- |
| Thesis advisor | Deisseroth, Karl |
| Thesis advisor | El Gamal, Abbas A |
| Advisor | Deisseroth, Karl |
| Advisor | El Gamal, Abbas A |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Laurie Davenport Burns. |
|---|---|
| Note | Submitted to the Department of Applied Physics. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2012. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2012 by Laurie Davenport Burns
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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