Identification and characterization of genes that affect the coordination of cell division with DNA replication in Bacillus subtilis
Abstract/Contents
- Abstract
- Dividing cells must ensure that their DNA is accurately replicated and that both copies of the chromosome are faithfully segregated into the new daughter cells before cell division completes. Here, I describe the identification and characterization of mechanisms that contribute to the coordination of cell division with DNA replication in the Gram-positive soil bacterium Bacillus subtilis. I developed a genetic screen to isolate mutations that increase the frequency with which B. subtilis cells erroneously divide over their chromosome when DNA replication is perturbed. Seventeen mutations were isolated in genes involved in a range of cellular processes including DNA repair, chromosome organization, cell wall structure, and central carbon metabolism. Six mutations affecting cell cycle coordination in B. subtilis were characterized, including detailed studies of the mechanism by which the DNA translocase SftA promotes chromosome separation at nascent division septa. I also investigated the role of YycJ, a putative metallo-beta-lactamase, in regulating cell division.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2010 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Biller, Steven James |
|---|---|
| Associated with | Stanford University, Department of Biological Sciences |
| Primary advisor | Burkholder, William |
| Thesis advisor | Burkholder, William |
| Thesis advisor | Grossman, Arthur (Arthur R.) |
| Thesis advisor | Hanawalt, Philip C, 1931- |
| Thesis advisor | Spormann, Alfred M |
| Advisor | Grossman, Arthur (Arthur R.) |
| Advisor | Hanawalt, Philip C, 1931- |
| Advisor | Spormann, Alfred M |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Steven James Biller. |
|---|---|
| Note | Submitted to the Department of Biology. |
| Thesis | Ph.D. Stanford University 2010 |
| Location | electronic resource |
Access conditions
- Copyright
- © 2010 by Steven James Biller
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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