Imaging data for "Beyond the MUN domain, Munc13 controls priming and depriming of synaptic vesicles"

<a href="https://embed.stanford.edu/iframe/?url=https%3A%2F%2Fpurl.stanford.edu%2Fwc823yz5804" class="su-underline">Show Content</a>

Abstract/Contents

Abstract: Synaptic vesicle docking and priming are dynamic processes. At the molecular level, SNAREs, synaptotagmins, Munc18, Munc13, and other factors are critical for Ca2+-triggered synaptic vesicle fusion with the plasma membrane, while disassembly factors, including the AAA+ ATPase N-ethylmalemide sensitive factor (NSF) and -SNAP disassemble and recycle SNAREs, but also antagonize fusion under some conditions. Here, we isolated synaptic vesicles from mouse brains and combined them with synthetic plasma membrane-mimicking vesicles in a new hybrid fusion assay. We included Munc18, Munc13, complexin, NSF, α-SNAP, and an ATP regeneration system and maintained their continuous presence—as in the neuron—to investigate how these opposing processes yield fusogenic synaptic vesicles. In this setting, synaptic vesicle association is reversible, and the ATP-regeneration system produces the most synchronous Ca2+-triggered fusion, suggesting that disassembly factors perform quality control at the early stages of synaptic vesicle association and establish a highly fusogenic state of the system. We uncovered a new functional role for Munc13 ancillary to the MUN domain that alleviates an α-SNAP-dependent inhibition of Ca2+-triggered fusion.

Author	Brunger, Axel	https://orcid.org/0000-0001-5121-2036 (unverified)
Author	Leitz, Jeremy	https://orcid.org/0009-0009-9976-2497 (unverified)

Subject	synaptic vesicle priming
Subject	neurotransmitter release
Subject	synaptic vesicle isolation
Subject	presynaptic protein architecture
Subject	SNARE
Subject	NSF
Subject	complexin
Subject	Munc18
Subject	Munc13
Subject	alpha-SNAP
Subject	single vesicle fusion assay
Genre	Data
Genre	Image
Genre	Data sets
Genre	Dataset

Related item	Preferred citation Jeremy Leitz, Chuchu Wang, Luis Esquivies, Richard A. Pfuetzner, John Jacob Peters, Sergio Couoh-Cardel, Austin L. Wang, Axel T. Brunger. Beyond the MUN domain, Munc13 controls priming and depriming of synaptic vesicles. Cell Reports, https://doi.org/10.1016/j.celrep.2024.114026 (2024).
DOI	https://doi.org/10.25740/wc823yz5804, https://doi.org/10.25740/wc823yz5804
Location	https://purl.stanford.edu/wc823yz5804

Use and reproduction: User agrees that, where applicable, content will not be used to identify or to otherwise infringe the privacy or confidentiality rights of individuals. Content distributed via the Stanford Digital Repository may be subject to additional license and use restrictions applied by the depositor.
License: This work is licensed under a Creative Commons Attribution 4.0 International license (CC BY).

Preferred citation: Brunger, A. (2024). Imaging data for "Beyond the MUN domain, Munc13 controls priming and depriming of synaptic vesicles". Stanford Digital Repository. Available at https://purl.stanford.edu/wc823yz5804. https://doi.org/10.25740/wc823yz5804.

Stanford Research Data

Loading usage metrics...