A forward genetic screen identifies new host factors influencing bacteriophage lambda's lysis-lysogeny decision
Abstract/Contents
- Abstract
- In order to survive, organisms must integrate internal and external signals via complex signaling pathways to intelligently navigate their environment. Bacteriophage lambda has long been a classic model for understanding such pathways, and how they allow cells to effect changes in physiology in order to adapt to varying circumstances. When infecting its host Escherichia coli, phage lambda may either make more viruses (lysis), or integrate itself into the host genome (lysogeny). To better understand the extent to which host physiology influences the lysis-lysogeny decision, I performed a forward genetic screen to identify genes whose deletions bias the infected cell towards lysis or lysogeny. This screen revealed numerous host-encoded genes linked to the lysis-lysogeny decision, including transcription factors and genes in metabolic pathways. These results demonstrate previously unknown links between host physiology and viral decision-making, shedding new light on this classic model system.
Description
| Type of resource | text |
|---|---|
| Date created | April 2018 |
Creators/Contributors
| Author | Quach, Nicolas Tung-Da |
|---|---|
| Primary advisor | Covert, Markus |
| Advisor | Brennan, Megan |
| Degree granting institution | Stanford University, Department of Bioengineering |
Subjects
| Subject | Bioengineering |
|---|---|
| Subject | Stanford |
| Subject | School of Engineering |
| Subject | systems biology |
| Subject | bacteriophage lambda |
| Subject | genetic screen |
| Genre | Thesis |
Bibliographic information
Access conditions
- Use and reproduction
- User agrees that, where applicable, content will not be used to identify or to otherwise infringe the privacy or confidentiality rights of individuals. Content distributed via the Stanford Digital Repository may be subject to additional license and use restrictions applied by the depositor.
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
Preferred citation
- Preferred Citation
- Quach, Nicolas Tung-Da. (2018). A forward genetic screen identifies new host factors influencing bacteriophage lambda's lysis-lysogeny decision. Stanford Digital Repository. Available at: https://purl.stanford.edu/vv414pn0268
Collection
Undergraduate Theses, School of Engineering
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- Contact
- nquach@stanford.edu
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