Identification of proteins at the parasitophorous vacuole membrane of Toxoplasma gondii
Abstract/Contents
- Abstract
- Toxoplasma gondii is an obligate intracellular parasite that replicates within a parasitophorous vacuole inside a host cell. The membrane encompassing this vacuole, the parasitophorous vacuole membrane (PVM), is extensively modified by Toxoplasma through the addition of various effector proteins. These effectors enable a variety of host-parasite interactions to occur at this interface that both support and inhibit the growth of the parasites. Despite the importance of the variety of known host-parasite interactions occurring at the PVM, only a small number have thus far been identified due to a poor understanding of the proteins present at this interface. Given this, the work described in this dissertation addresses two important research topics. First, the study presented in Chapter 2 uses affinity purification of the Toxoplasma PVM protein MYR1 followed by mass spectrometry to identify and functionally characterize additional proteins involved in the translocation of Toxoplasma effectors across the PVM, a critical mechanism by which Toxoplasma can influence and reshape the host environment. Second, the work presented in Chapter 3 identifies host and parasite proteins specifically present at the host cytosolic side of the PVM using recent advances in enzyme-catalyzed protein proximity labeling. This method takes advantage of the promiscuous biotin ligase miniTurbo to label proteins in its immediate vicinity. This is the first known study designed to identify all proteins on the host cytosolic side of the PVM in an unbiased way. The knowledge gained from these two approaches provides a substantial advance in our understanding of, and ability to mechanistically study, interesting and disease-relevant aspects of host-parasite biology occurring at the literal interface of Toxoplasma and the host cells it infects.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2020; ©2020 |
| Publication date | 2020; 2020 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Cygan, Alicja Maria |
|---|---|
| Degree supervisor | Boothroyd, John C |
| Thesis advisor | Boothroyd, John C |
| Thesis advisor | Carette, Jan, 1971- |
| Thesis advisor | Egan, Elizabeth S |
| Thesis advisor | Kim, Peter, 1958- |
| Degree committee member | Carette, Jan, 1971- |
| Degree committee member | Egan, Elizabeth S |
| Degree committee member | Kim, Peter, 1958- |
| Associated with | Stanford University, Department of Microbiology and Immunology |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Alicja Maria Cygan. |
|---|---|
| Note | Submitted to the Department of Microbiology and Immunology. |
| Thesis | Thesis Ph.D. Stanford University 2020. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2020 by Alicja Maria Cygan
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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