Designing cell-instructive granular inks for 3D bioprinting
Abstract/Contents
- Abstract
- Hydrogel-based biomaterials have seen widespread use in tissue engineering and 3D bioprinting due to their extensive tunability, which enables the design of matrices with a broad range of application-specific mechanical and biological characteristics. Despite this, the viscoelastic requirements of 3D bioprinting make it difficult to design hydrogel-based biomaterial inks that maintain printability while effectively promoting cell function. Granular hydrogels composed of jammed hydrogel microparticles (termed 'microgels') have recently garnered significant attention for their potential to solve this challenge. As cell culture platforms, granular hydrogels may readily provide microenvironmental signals proven to regulate cell behavior. Meanwhile, the macroscale properties of granular hydrogels naturally tend to meet the viscoelastic requirements of extrusion-based bioprinting. In this thesis, I explore strategies for addressing current limitations in designing biomaterial inks to influence endothelial cell behavior within printed constructs. To that end, I present two families of multi-component granular inks that leverage microgel modularity to achieve desirable printability, microarchitecture, and cell response. Through this work, I demonstrate the potential for modular, microgel-based inks to address longstanding challenges in the bioprinting of functional tissues.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2023; ©2023 |
| Publication date | 2023; 2023 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Seymour, Alexis Jane |
|---|---|
| Degree supervisor | Heilshorn, Sarah |
| Thesis advisor | Heilshorn, Sarah |
| Thesis advisor | Skylar-Scott, Mark |
| Thesis advisor | Yang, Fan, 1975- |
| Degree committee member | Skylar-Scott, Mark |
| Degree committee member | Yang, Fan, 1975- |
| Associated with | Stanford University, School of Engineering |
| Associated with | Stanford University, Department of Bioengineering |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Alexis J. Seymour. |
|---|---|
| Note | Submitted to the Department of Bioengineering. |
| Thesis | Thesis Ph.D. Stanford University 2023. |
| Location | https://purl.stanford.edu/vs244tk9631 |
Access conditions
- Copyright
- © 2023 by Alexis Jane Seymour
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
Also listed in
Loading usage metrics...