Low pH effects on early development and toxin transporter activity in sea urchins (Strongylocentrotues purpuratus, Tripneustes gratilla, Echinometra mathaei)

<a href="https://embed.stanford.edu/iframe/?url=https%3A%2F%2Fpurl.stanford.edu%2Fvq436sq9834" class="su-underline">Show Content</a>

Abstract/Contents

Abstract: This study explores both the direct effects of ocean acidification on early embryology and the indirect effects of a low pH environment on the embryos ability to expel toxins through membrane-bound transporters. This work was done on three different species of sea urchins, Strongylocentrotus Purpuratus, Tripneustes gratilla, and Echinometra mathaei, each collected at a different location (Monterey, CA, Hawaii, and Washington Island, respectively). Cell counts in individuals were performed to assess impact on early development. Surface membrane toxin transporters (mrp, pgp) were blocked, and accumulation of a toxin proxy, calcein-am, was noted by measuring the fluorescent levels as calcein-am hydrolyzed into fluorescent calcein in the embryo. The results indicate negative effects of low pH on early development of Echinometra, while the toxin transporters in all three species appear to work more effectively at low pH. There is some evidence that specific types of transporters may work more effectively until a threshold pH, below where the transporter no longer functions as well. In general, Echinometra had less transporter activity than Tripneustes.

Type of resource	text
Date created	June 11, 2007

Author	Rizk, Sarah

Subject	ocean acidification
Subject	embryology
Subject	pH
Subject	embryo
Subject	toxins
Subject	membrane-bound transporter
Subject	urchin
Subject	Strongylocentrotus purpuratus
Subject	Tripneustes gratilla
Subject	Echinometra mathaei
Subject	Monterey
Subject	California
Subject	Hawaii
Subject	Washington Island
Subject	mrp
Subject	pgp
Subject	calcein-am
Subject	fluorescence
Genre	Student project report

Location	https://purl.stanford.edu/vq436sq9834

Use and reproduction: User agrees that, where applicable, content will not be used to identify or to otherwise infringe the privacy or confidentiality rights of individuals. Content distributed via the Stanford Digital Repository may be subject to additional license and use restrictions applied by the depositor.
License: This work is licensed under a Creative Commons Attribution 3.0 Unported license (CC BY).

Preferred Citation: Rizk, Sarah. 2007. Low pH effects on early development and toxin transporter activity in sea urchins (Strongylocentrotues purpuratus, Tripneustes gratilla, Echinometra mathaei). Unpublished Student Work, S-211, Stanford@SEA, Stanford Digital Repository. https://purl.stanford.edu/vq436sq9834.

Stanford@SEA -- Student papers

Loading usage metrics...