A Bacterial Surface Layer Protein Exploits Multi-step Crystallization for Rapid Self-assembly
Abstract/Contents
- Abstract
- Surface layers (S-layers) are crystalline protein coats surrounding microbial cells. S-layer proteins (SLPs) regulate their extracellular self-assembly by crystallizing when exposed to an environmental trigger. However, molecular mechanisms governing rapid protein crystallization in vivo or in vitro are largely unknown. Here, we demonstrate that the C. crescentus SLP readily crystallizes into sheets in vitro via a calcium-triggered multi-step assembly pathway. This pathway involves two domains serving distinct functions in assembly. The C-terminal crystallization domain forms the physiological 2D crystal lattice, but full-length protein crystallizes multiple orders of magnitude faster due to the N-terminal nucleation domain. Observing crystallization using a time course of electron cryo-microscopy (Cryo-EM) imaging reveals a crystalline intermediate wherein N-terminal nucleation domains exhibit motional dynamics with respect to rigid lattice-forming crystallization domains. Dynamic flexibility between the two domains rationalizes efficient S-layer crystal nucleation on the curved cellular surface. Rate enhancement of protein crystallization by a discrete nucleation domain may enable engineering of kinetically controllable self-assembling 2D macromolecular nanomaterials.
Description
| Type of resource | software, multimedia |
|---|---|
| Date created | October 21, 2019 |
Creators/Contributors
| Author | Herrmann, Jonathan | |
|---|---|---|
| Author | Li, Po-Nan | |
| Author | Jabbarpour, Fatemeh | |
| Author | Chan, Anson C.K. | |
| Author | Rajkovic, Ivan | |
| Author | Matsui, Tsutomu | |
| Author | Shapiro, Lucy | |
| Author | Smit, John | |
| Author | Weiss, Thomas | |
| Author | Murphy, Michael E.P. | |
| Author | Wakatsuki, Soichi |
Subjects
| Subject | protein self-assembly |
|---|---|
| Subject | Cryo-EM time course |
| Subject | microbiology |
| Subject | biophysics |
| Genre | Dataset |
Bibliographic information
| Related Publication | bioRxiv 665745; doi: https://doi.org/10.1101/665745 |
|---|---|
| Location | https://purl.stanford.edu/tz311fw8492 |
Access conditions
- Use and reproduction
- User agrees that, where applicable, content will not be used to identify or to otherwise infringe the privacy or confidentiality rights of individuals. Content distributed via the Stanford Digital Repository may be subject to additional license and use restrictions applied by the depositor.
Preferred citation
- Preferred Citation
- Soichi, Wakatsuki. (2019). A Bacterial Surface Layer Protein Exploits Multi-step Crystallization for Rapid Self-assembly. Stanford Digital Repository. Available at: https://purl.stanford.edu/tz311fw8492
Collection
Stanford Research Data
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- Contact
- soichi.wakatsuki@stanford.edu
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