The role of DNA endoreduplication in ABA's regulation of root growth in Arabidopsis thaliana
Abstract/Contents
- Abstract
- Climate change is expected to bring shifts in global precipitation, and in areas where water availability is limiting, adaptations to water-deficit stress are essential. Roots play an essential role in acquiring nutrients and water necessary for plant plant growth, and recent research has shown that root growth patterns during periods of water stress can be highly variable within taxonomic groups. Abscisic acid (ABA) is a stress-response hormone that has been demonstrated to be an important regulator of root growth, and there is evidence that increasing ploidy in root cells, a process called endoreduplication, may increase root extension by enlarging root cell size. However, the pathway through which ABA regulates root endoreduplication processes is still unclear. The purpose of this study is to investigate the divergence of the endoreduplication pathway in regulating root growth response to stress in the Brassicaceae family. For this project, DAPI staining and confocal fluorescence microscopy protocols were developed for quantification of nuclear area. Transgenic mutants were then used to identify the ABA-driven response of genes regulating endoreduplication in the model species Arabidopsis thaliana. Identifying the role of ABA in root developmental processes provides a better understanding of changes in root growth in response to environmental factors such as water stress. Furthermore, this research has the potential to inform new methods of plant genetic modification that support the goal of establishing new plant varieties better suited to the changing conditions of the anthropocene
Description
| Type of resource | text |
|---|---|
| Date modified | December 5, 2022 |
| Publication date | May 6, 2022; May 2022 |
Creators/Contributors
| Author | Camacho, Nicholas |
|---|---|
| Thesis advisor | Dinneny, José |
| Thesis advisor | Fukami, Tadashi |
| Degree granting institution | Stanford University, Department of Biology |
Subjects
| Subject | Water stress |
|---|---|
| Subject | Biology |
| Subject | Abscisic acid |
| Subject | Roots (Botany) > Growth |
| Subject | Arabidopsis thaliana |
| Subject | Endoreduplication |
| Genre | Text |
| Genre | Thesis |
Bibliographic information
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- This work is licensed under a Creative Commons Attribution Non Commercial 4.0 International license (CC BY-NC).
Preferred citation
- Preferred citation
- Camacho, N. and Dinneny, J. (2022). The role of DNA endoreduplication in ABA's regulation of root growth in Arabidopsis thaliana. Stanford Digital Repository. Available at https://purl.stanford.edu/tw765ch3214
Collection
Undergraduate Theses, Department of Biology, 2021-2022
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- Contact
- camacho1@stanford.edu
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