Microfluidic protein bioassays using giant magnetoresistive sensors
Abstract/Contents
- Abstract
- For high performance medical diagnostics, magnetic biosensors have emerged as a suitable platform for fast and accurate protein measurements due to their very low background signal compared to optical methods. Medical applications demand biomarker panels including a larger number of protein analytes, and biosensors with larger and larger numbers of individual sensors enable measuring multiple target analytes in parallel on the same chip. Of these biosensors, Giant Magnetoresistance (GMR) sensors have been identified as one of the most promising technologies for protein detection. As with any solid state biosensor technology, on GMR sensors the effective use of each sensor is limited by its dynamic range and cross-reactivity between analytes used. Each added analyte added onto the sensor needs to be matched in terms of dynamic range of the sensor and using only reagents that do not cross react with other assay reagents. These two constraints limit the target analyte multiplexing capability of the sensor array technology. In order to use large-scale GMR sensor arrays effectively, these constraints need to be overcome. This thesis presents a microfluidic integration approach to GMR sensors which interfaces groups of sensors using individual microfluidic channels as separate compartments and thereby limits the scope of these constraints to only the compartment level and not to the whole array. In addition to overcoming dynamic range and cross-reactivity limitations in protein assays, this compartmentalization approach increases the number of samples that can be measured in parallel on a single chip from 1 up to 8 and enables more precise protein quantification. The use of microfluidics further increases the snr of larger particles with increased magnetic moments. These particles lead to an order of magnitude improvement in detection limit. Furthermore this microfluidic approach enables the integration of sample and reagent reservoirs, actuation elements and automation within the microfluidic chip. Microfluidic compartmentalization is useful for enabling an effective and flexible use of a wide range of surface-based sensor arrays. It enables the deployment of large-scale sensor arrays, which are not limited by cross-reactivity and dynamic range.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2015 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Bechstein, Daniel Jacob Benjamin |
|---|---|
| Associated with | Stanford University, Department of Mechanical Engineering. |
| Primary advisor | Santiago, Juan G |
| Primary advisor | Wang, Shan |
| Thesis advisor | Santiago, Juan G |
| Thesis advisor | Wang, Shan |
| Thesis advisor | Pruitt, Beth |
| Thesis advisor | Quake, Stephen Ronald |
| Advisor | Pruitt, Beth |
| Advisor | Quake, Stephen Ronald |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Daniel Jacob Benjamin Bechstein. |
|---|---|
| Note | Submitted to the Department of Mechanical Engineering. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2015. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2015 by Daniel Jacob Benjamin Bechstein
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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