Proteomic single-synapse analysis with array tomography
Abstract/Contents
- Abstract
- Array tomography (AT) is a high-resolution proteomic imaging method that exploits a combination of light and EM techniques to resolve fine details at the synapse level across large fields of view spanning entire circuits. Much of my graduate work has centered on helping to shape AT into an imaging method fit for rigorous use, by developing computational algorithms which take advantage of AT's particular qualities to automate its operation. Among them are: a mapping tool for microscopy software to automate the imaging of AT's unique section layout; Multistackreg, an alignment plugin which is capable of registering multiple imaging sessions on the same piece of tissue; a cross-correlation algorithm for fluorescent channel validation; the synaptogram, a tool for visualizing synapse-scale high-dimensional proteomic molecular complexes; and an active learning paradigm for large-scale synapse quantification.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2011 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Busse, Brad Lee |
|---|---|
| Associated with | Stanford University, Biophysics Program. |
| Primary advisor | Smith, Stephen |
| Thesis advisor | Smith, Stephen |
| Thesis advisor | Hestrin, Shaul |
| Thesis advisor | Lewis, Richard (Richard Sheridan) |
| Thesis advisor | Luo, Liqun, 1966- |
| Advisor | Hestrin, Shaul |
| Advisor | Lewis, Richard (Richard Sheridan) |
| Advisor | Luo, Liqun, 1966- |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Brad Busse. |
|---|---|
| Note | Submitted to the Department of Biophysics. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2011. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2011 by Brad Lee Busse
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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