Proteomic imaging of synaptic change : using array tomography to screen for molecular components of barrel cortex map plasticity
Abstract/Contents
- Abstract
- Plasticity of connections within the neocortical microcircuit is thought to be the substrate for perceptual learning and memory. Mouse whisker barrel cortex offers many unique experimental advantages for the study of neocortical plasticity because of an accessible sensory periphery and a repeated, stereotyped columnar architecture. These features make it possible to characterize the microcircuitry of the cortical column, a potential common circuit element across species and cortical areas. I have developed an experimental and computational pipeline for characterizing the distribution of molecularly distinct synapse populations within whisker barrel columns using array tomography, a technique developed in our laboratory. I have performed preliminary explorations of modifications of the synaptic architecture of the mouse barrel cortex microcircuit produced by a brief period of sensory deprivation during early adulthood, and identify specific synapse populations most subject to such modification. I have also worked with collaborators at the Open Connectome Project to host this data and my software publicly online so that others can also mine it to explore the proteomic complexity of synaptic microcircuits.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2013 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Weiler, Nicholas Collins |
|---|---|
| Associated with | Stanford University, Neurosciences Program. |
| Primary advisor | Smith, Stephen |
| Thesis advisor | Smith, Stephen |
| Thesis advisor | Hestrin, Shaul |
| Thesis advisor | Raymond, Jennifer L |
| Thesis advisor | Shen, Kang, 1972- |
| Advisor | Hestrin, Shaul |
| Advisor | Raymond, Jennifer L |
| Advisor | Shen, Kang, 1972- |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Nicholas Collins Weiler. |
|---|---|
| Note | Submitted to the Program in Neurosciences. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2013. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2013 by Nicholas Collins Weiler
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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