Modular microring arrays for versatile in vitro recording
Abstract/Contents
- Abstract
- Electroactive cells such as neurons and myocytes enable all of the biological pro-cesses that allow us to do, sense, and think. Nondestructive in vitro recordings of these cells are critical to deciphering the fundamental processes underpinning all of human bi-ology, understanding and treating degenerative diseases such as epilepsy, and even dif-ferentiating patient-derived stem cells for tissue transplantation and personalized medi-cine. However, the recording quality of massively parallel electrode arrays continues to be bottlenecked by poor mechanical and electrochemical coupling between the cell and the recording electrode. A key breakthrough in the field has been moving beyond planar devices and toward three-dimensional nanostructures; however, most sub-micron elec-trodes suffer from prohibitively high impedances that limit their usefulness as a biologi-cal tool. Here, we describe a modular microelectrode that decouples the active electrode from the physical sealing mechanic to achieve a breakthrough combination of high leak re-sistance and low electrochemical impedance. These tuneable microring electrodes exhibit high sensitivity even at length scales below the size of a single neuron, and can electro-porate into mammalian neurons to obtain intracellular recordings. Moreover, they are the first chip-based devices capable of performing not only current clamp, but also voltage clamp experiments. This seal-promoting geometry can be combined with chemical func-tionalization to mimic cell-to-cell junctions at the electrode interface. Using protein pho-tolithography to selectively pattern N-cadherin onto our microring electrodes, we were able to achieve intracellular recordings from mouse cardiomyocytes without the aid of electroporation. These devices recorded intracellularly for over two continuous hours—the longest uninterrupted intracellular recording reported to date. By permitting truly non-perturbative recording, this platform will enable electrophysiologists to perform extend-ed recordings that are currently impossible with destructive patch clamp techniques.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2018; ©2018 |
| Publication date | 2018; 2018 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Chang, Katherine |
|---|---|
| Degree supervisor | Melosh, Nicholas A |
| Thesis advisor | Melosh, Nicholas A |
| Thesis advisor | Brongersma, Mark L |
| Thesis advisor | Heilshorn, Sarah |
| Degree committee member | Brongersma, Mark L |
| Degree committee member | Heilshorn, Sarah |
| Associated with | Stanford University, Department of Materials Science and Engineering. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Katherine Chang. |
|---|---|
| Note | Submitted to the Department of Materials Science and Engineering. |
| Thesis | Thesis Ph.D. Stanford University 2018. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2018 by Katherine Gloria Chang
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