The MHC class II cofactor, HLA-DM, interacts with immunoglobulin in B cells
Abstract/Contents
- Abstract
- B cells internalize antigen bound to membrane immunoglobulin and load antigen-derived peptides onto MHC class II proteins. How these pathways intersect is not well understood. We found that HLA-DM, a catalyst for peptide loading onto MHC class II, regulates immunoglobulin levels in human B cells and co-precipitates with immunoglobulin. In intact cells, HLA-DM and immunoglobulin are found in close proximity, and these complexes are in post-Golgi compartments. In vitro, in the endosomal pH range, the luminal domain of soluble HLA-DM (sDM) directly and specifically interacts with the immunoglobulin Fab domain, and sDM increases the amount of antigen released from immune complexes. These observations support a model in which HLA-DM facilitates the hand-off of antigen from immunoglobulin to MHC class II.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2013 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Strohman, Michael John |
|---|---|
| Associated with | Stanford University, Program in Immunology. |
| Primary advisor | Mellins, Elizabeth |
| Thesis advisor | Mellins, Elizabeth |
| Thesis advisor | Davis, Mark M |
| Thesis advisor | Jardetzky, Theodore |
| Thesis advisor | Jones, Patricia P |
| Thesis advisor | Miklos, David (David B.) |
| Advisor | Davis, Mark M |
| Advisor | Jardetzky, Theodore |
| Advisor | Jones, Patricia P |
| Advisor | Miklos, David (David B.) |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Michael John Strohman. |
|---|---|
| Note | Submitted to the Program in Immunology. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2013. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2013 by Michael John Strohman
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