Supplementary data on macrophage cells for thesis entitled: "Advances on chip inside a cell for monitoring physiological cell parameters"

Continuous monitoring of physiological parameters inside a living cell will lead to major advances in understanding of biology and complex diseases, such as cancer. It also enables us to develop new medical diagnostics and therapeutics. In addition, progress in nanofabrication and wireless communication has opened up the potential of making our wireless chip small enough that it can be wholly inserted into a cell. To investigate how the chip could be internalized into the cell and how the chip would affect cell physiology, we designed and fabricated a series of 3D multilayer structures with different sizes as a potential RFID (Radio Frequency IDentification) cell tracker. Our experiments show that the chips with smaller sizes than 21 micron x 9 micron x 1.5 micron can be easily internalized by various types of living cells, such as macrophages, cancer cells, and normal/healthy cells. The incubated cells with internalized chips stayed alive during the 5 days of monitoring. Also, we observed successful cell division from these incubated cells. These results are the first steps towards long-term, wireless, intracellular physiologic monitoring.

This data set contains short and long videos of cell division or internalization (as indicated in the file names) of macrophages cells incubating microscale silicon chips of 4 different sizes -- 12 micron x 12 micron, 9 micron x 15 micron, 9 micron x 18 micron, and 9 micron x 21 micron.