Interrogating, manipulating, and controlling nano-bio interfaces
Abstract/Contents
- Abstract
- Cells communicate through direct contact and soluble chemical signals. Mimicking an extracellular environment requires controlling these signals at micron length scales. Integrated circuits make electronic control at these scales trivial, but fluidic control at these length scales requires very different principles. Standard microfluidic devices can finely control flowing fluids, but fluid flow affects cells in a myriad of ways. Alternatively, diffusion based chemical delivery methods tend to be crude, ill defined systems that offer very limited control. This thesis describes three distinctive platforms that combine the active spatial and temporal control of microfluidic systems with a delivery system that relies purely on diffusion. First, we detail a silicon based array of nanoreservoirs underneath the cell culture surface which are used to store and release bioactive molecules. These reservoirs are opened and closed with electrochemical dissolution and deposition at a narrow reservoir opening. Next, we describe an adaptation of traditional, elastomer based microfluidics. In these devices the cell culture area is separated from a microfluidic channel located directly underneath the chamber by a nanoporous membrane. The desirable microfluidic properties, including temporal and spatial control, are preserved, while fluidic flow over the cells is eliminated. Finally, we demonstrate a novel "nanostraw" culture surface, which is combined with the previous device to offer fluidic access directly to the cell cytosol, creating a powerful tool with implications for cell delivery and sampling. Additional work on probing the assembly of protein structures is also detailed. Clathrin 2-dimensional lattice assembly on lipid monolayers, serving as cell membrane mimics, was monitored and studied through surface rheological techniques. Rheological measurements elucidated important network properties, and the formation process was compared to various models for clathrin network assembly.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2011 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | VanDersarl, Jules John |
|---|---|
| Associated with | Stanford University, Department of Materials Science and Engineering |
| Primary advisor | Melosh, Nicholas A |
| Thesis advisor | Melosh, Nicholas A |
| Thesis advisor | Heilshorn, Sarah |
| Thesis advisor | Longaker, Michael T |
| Advisor | Heilshorn, Sarah |
| Advisor | Longaker, Michael T |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Jules J. VanDersarl. |
|---|---|
| Note | Submitted to the Department of Materials Science and Engineering. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2011. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2011 by Jules John VanDersarl
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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