Modeling cardiovascular risks and diseases using human induced pluripotent stem cells and tissue engineering
Abstract/Contents
- Abstract
- Cell-based, in vitro models of the cardiovascular system are invaluable for enhancing our understanding of cardiovascular health and disease. In recent years, the development of induced pluripotent stem cell (iPSC) technology has enabled effective modeling of cardiovascular diseases. In the first part of my thesis work, we utilized iPSC-derived cardiomyocytes (iPSC-CMs) to investigate the effects of microgravity on cardiomyocyte function and gene expression. This study represented both the first implementation of long-term human cell culture in space and the first use of iPSC technology to model the effects of spaceflight on human cardiomyocyte structure and function. The major drawback of using iPSC-CMs for cardiovascular modeling is their immature, fetal-like phenotype. In the second part of my thesis work, we utilized EHTs to enhance iPSC-CM maturation and further validate the results from two iPSC-CM studies that investigated (1) the mechanism by which a mutation in MYBPC3, a contractile protein, causes hypertrophic cardiomyopathy and (2) patient-specific responses to calcium channel blockers, a class of drugs commonly used to treat hypertension. In the final part of my thesis work, we utilized a sheep carotid interposition graft model to investigate whether a biodegradable external sheath could reduce graft maladaptation and failure. Collectively, this work describes the use of animal models, iPSCs, and tissue engineering to effectively model cardiovascular risks, disease, and drug responses.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2021; ©2021 |
| Publication date | 2021; 2021 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Wnorowski, Alexa |
|---|---|
| Degree supervisor | Wu, Joseph Ching-Ming, 1971- |
| Thesis advisor | Wu, Joseph Ching-Ming, 1971- |
| Thesis advisor | Marsden, Alison (Alison Leslie), 1976- |
| Thesis advisor | Woo, Joseph |
| Degree committee member | Marsden, Alison (Alison Leslie), 1976- |
| Degree committee member | Woo, Joseph |
| Associated with | Stanford University, Department of Bioengineering |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Alexa Wnorowski. |
|---|---|
| Note | Submitted to the Department of Bioengineering. |
| Thesis | Thesis Ph.D. Stanford University 2021. |
| Location | https://purl.stanford.edu/sy883xk4496 |
Access conditions
- Copyright
- © 2021 by Alexa Wnorowski
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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