Biochemical, electrical, and optogenetic control of human pluripotent stem cell-derived cardiomyocytes
Abstract/Contents
- Abstract
- Cardiovascular disease affects more than 70 million Americans and is the number one cause of mortality in the United States. Because the regenerative capacity of cardiac tissue is limited, human pluripotent stem cells (hPSC) have emerged as a potential source for cellular-based therapies. However, for these therapies to be effective, sufficient amounts of differentiated cells must be produced, these cells must be identified and sorted, and, upon implantation, arrhythmias must be avoided. In this dissertation, I describe the biochemical control of hPSC for their directed differentiation into cardiomyocytes, electrical control for electrophysiology-based cell sorting, and optogenetic control for temporal synchronization. For future therapy, the in vivo application of optical stimulation could allow immediate, precise, and specific synchronization of efficiently derived and purified hPSC-CM with patient cardiac rates and rhythms. This, in turn, would significantly reduce the chance of arrhythmias arising from implanted hPSC-CM, and, therefore, contribute towards establishing a safe and effective cell-based therapy.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2012 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Abilez, Oscar John |
|---|---|
| Associated with | Stanford University, Department of Bioengineering. |
| Primary advisor | Zarins, Christopher K |
| Thesis advisor | Zarins, Christopher K |
| Thesis advisor | Quake, Stephen Ronald |
| Thesis advisor | Wu, Joseph Ching-Ming, 1971- |
| Thesis advisor | Yock, Paul G |
| Advisor | Quake, Stephen Ronald |
| Advisor | Wu, Joseph Ching-Ming, 1971- |
| Advisor | Yock, Paul G |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Oscar John Abilez. |
|---|---|
| Note | Submitted to the Department of Bioengineering. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2012. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2012 by Oscar John Abilez
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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