In vitro analysis of the complete fatty acid biosynthetic pathway from Escherichia coli and implications on metabolic engineering of E. coli for the production of fatty acid based biofuels
Abstract/Contents
- Abstract
- With increasing demands of energy, depletion of global fossil fuel reserves, and unsustainable emission rates of greenhouse gases, there is considerable impetus for the development of renewable fuel sources. Fatty acid derived biofuels, being energy-dense and compatible with existing infrastructure, represent a promising supplement to petroleum-based fuels. Earlier work has led to extensive genetic and biochemical characterization of the fatty acid biosynthesis pathway in Escherichia coli. More recently, the potential of using E. coli as a biocatalyst for converting biomass into fatty acid derivatives in an economically viable manner has been explored. The dissertation focuses on in vitro analysis of the complete fatty acid biosynthetic pathway from E. coli, and discusses strategies for enhancing biosynthesis of fatty acids and derivatives in vivo based on findings from the in vitro analysis. The entire pathway has been successfully reconstituted in vitro, providing a powerful starting point to understand and engineer rate-limiting and regulatory steps in the complicated pathway, thereby rationally guiding future metabolic engineering efforts for E. coli -- catalyzed biofuel production.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2012 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Yu, Xingye |
|---|---|
| Associated with | Stanford University, Department of Chemical Engineering |
| Primary advisor | Khosla, Chaitan, 1964- |
| Thesis advisor | Khosla, Chaitan, 1964- |
| Thesis advisor | Spormann, Alfred M |
| Thesis advisor | Swartz, James R |
| Advisor | Spormann, Alfred M |
| Advisor | Swartz, James R |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Xingye Yu. |
|---|---|
| Note | Submitted to the Department of Chemical Engineering. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2012. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2012 by Xingye Yu
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