Functional analysis of YneA, an SOS-induced inhibitor of cell division in Bacillus subtilis
Abstract/Contents
- Abstract
- In both prokaryotic and eukaryotic organisms, the coordination of cell division with DNA replication, segregation, and repair is critical for ensuring that each daughter cell receives an intact copy of the genome. In Bacillus subtilis, DNA damage triggers the SOS response, which activates a host of repair pathways, as well as an inhibitor of cell division, YneA. The mechanism by which YneA inhibits cell division and the processes involved in shutting off YneA activity remain unclear. In this work, we show that YneA is induced under a variety of DNA stress conditions, including replication initiation blocks. We observed that YneA is exported, does not stably associate with the cell wall, and is rapidly proteolyzed. Our results also suggest that YneA may be active prior to signal peptide cleavage. We have isolated mutations that indicate that protein-protein interactions mediated by the transmembrane domain might be required for YneA activity. One possible YneA target may be DivIB, a divisome component that we identified in a screen for suppressors of yneA overexpression.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2010 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Mo, Allison H |
|---|---|
| Associated with | Stanford University, Department of Biological Sciences |
| Primary advisor | Burkholder, William |
| Thesis advisor | Burkholder, William |
| Thesis advisor | Cyert, Martha S, 1958- |
| Thesis advisor | Mudgett, Mary Beth, 1967- |
| Thesis advisor | Stearns, Tim |
| Advisor | Cyert, Martha S, 1958- |
| Advisor | Mudgett, Mary Beth, 1967- |
| Advisor | Stearns, Tim |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Allison Huei-Juin Mo. |
|---|---|
| Note | Submitted to the Department of Biological Sciences. |
| Thesis | Ph.D. Stanford University 2010 |
| Location | electronic resource |
Access conditions
- Copyright
- © 2010 by Allison H. Mo
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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