Cytokine receptor engineering for mechanistic and therapeutic studies
Abstract/Contents
- Abstract
- The activity of immune cells is regulated by proteins on the cell surface called cytokine receptors. These proteins allow cells to grow in a controlled manner and to balance immunosuppressive and stimulatory activities. My dissertation has focused on engineering cytokine receptors to understand their function and to target cells of interest therapeutically. I first focused on the interleukin-2 receptor (IL-2R), both to engineer orthogonal activity for selective cell growth, and to determine how unique signals are transmitted when binding to interleukin-15 (IL-15) and IL-15 receptor (IL-15R) complexes. I then focused on the colony-stimulating factor 3 receptor (CSF3R) which controls the growth of neutrophils, the most abundant type of white blood cell. I investigated if a mutated CSF3R epitope could be targeted therapeutically, why this amino acid substitution is the predominant mutation in chronic neutrophilic leukemia, and how it leads to uncontrolled neutrophil growth. Combined, these engineering approaches highlight the function and therapeutic potential of cytokine receptors.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2022; ©2022 |
| Publication date | 2022; 2022 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Hollander, Michael Jacob |
|---|---|
| Degree supervisor | Bertozzi, Carolyn R, 1966- |
| Degree supervisor | Cochran, Jennifer R |
| Thesis advisor | Bertozzi, Carolyn R, 1966- |
| Thesis advisor | Cochran, Jennifer R |
| Thesis advisor | Gotlib, Jason |
| Thesis advisor | Huang, Possu |
| Degree committee member | Gotlib, Jason |
| Degree committee member | Huang, Possu |
| Associated with | Stanford University, Department of Bioengineering |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Michael Jacob Hollander. |
|---|---|
| Note | Submitted to the Department of Bioengineering. |
| Thesis | Thesis Ph.D. Stanford University 2022. |
| Location | https://purl.stanford.edu/sp070fc2856 |
Access conditions
- Copyright
- © 2022 by Michael Jacob Hollander
- License
- This work is licensed under a Creative Commons Attribution 3.0 Unported license (CC BY).
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