Targeting lymphoma with precision using semi-synthetic anti-idiotype peptibodies
Abstract/Contents
- Abstract
- B-cell lymphomas express a functionally active and truly tumor-specific cell-surface product, the variable region of the B-cell receptor, otherwise known as idiotype. The tumor idiotype differs from patient to patient, however, making it a technical challenge to exploit for therapy. We have developed a method of targeting idiotype using a semi-synthetic personalized therapeutic that is more practical to produce on a patient-by-patient basis than monoclonal antibodies. In this method, a small peptide with affinity for a tumor idiotype is identified by screening a library, chemically synthesized, and then affixed to the amino-terminus of a pre-made IgG Fc protein. We demonstrate that the resultant semi-synthetic anti-idiotype peptibodies kill tumor cells in vitro with specificity, trigger tumor cell phagocytosis by macrophages, and efficiently clear human lymphoma in a murine xenograft model. Our results suggest that this method could be used to target tumor with true precision on a personalized basis.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2015 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Torchia, James Anthony |
|---|---|
| Associated with | Stanford University, Interdepartmental Program in Immunology. |
| Primary advisor | Levy, Ronald, 1941 December 6- |
| Thesis advisor | Levy, Ronald, 1941 December 6- |
| Thesis advisor | Alizadeh, Ash |
| Thesis advisor | Weissman, Irving L |
| Advisor | Alizadeh, Ash |
| Advisor | Weissman, Irving L |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | James Torchia. |
|---|---|
| Note | Submitted to the Interdepartmental Program in Immunology. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2015. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2015 by James Anthony Torchia
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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