Far from home : understanding pleiotropic effects of adaptive mutations in non-home environments
Abstract/Contents
- Abstract
- Understanding how organisms adapt to their environment and determining the fitness consequences of such adaptation is a major goal of evolutionary biology. It is unknown how mutations that are adaptive in one environment fare in a new environment. The fitness consequences of mutations in many different environments is what we define as pleiotropy. In this thesis, I shed light on adaptive mutations in the environment they evolved in (or "home" environment) and their effects in novel environments ("non-home" environments). First, I characterize components of the morphological adaptation of the dynamic pyrenoid in the algae, Chlamydomonas reinhardtii in both low carbon concentrations ("home environment") and high carbon concentrations ("non-home environment"). Second, I leverage barcode lineage tracking to analyze individual adaptive mutations from both haploid and diploid evolved populations and estimate their fitness effects in both their home and non-home environments. Lastly, I identified adaptive mutations and constructed homozygous and heterozygous mutant strains to gain insight into the roles of dominance and pleiotropy in adaptation. I find that environment is not the sole driver of pleiotropy, but ploidy, dominance, and target genes contribute to fitness in other environments. Together, this study of adaptive mutations reveals a diversity of forces driving pleiotropy that are hidden and cannot be revealed by the mutation's fitness in its home environment alone.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2022; ©2022 |
| Publication date | 2022; 2022 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Chen, Vivian Kien |
|---|---|
| Degree supervisor | Sherlock, Gavin |
| Thesis advisor | Sherlock, Gavin |
| Thesis advisor | Cyert, Martha S, 1958- |
| Thesis advisor | Good, Benjamin H |
| Thesis advisor | Petrov, Dmitri Alex, 1969- |
| Degree committee member | Cyert, Martha S, 1958- |
| Degree committee member | Good, Benjamin H |
| Degree committee member | Petrov, Dmitri Alex, 1969- |
| Associated with | Stanford University, Department of Biology |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Vivian Kien Chen. |
|---|---|
| Note | Submitted to the Department of Biology. |
| Thesis | Thesis Ph.D. Stanford University 2022. |
| Location | https://purl.stanford.edu/ry347gb0055 |
Access conditions
- Copyright
- © 2022 by Vivian Kien Chen
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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