Antibody approaches to enable hematopoietic stem cell and organ transplant

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Clinical care has been greatly advanced by precision medicine, which has been represented by antibodies, chimeric antigen receptor T-cells, small molecule inhibitors to mutant proteins, and many other therapies. The mainstay of this progress has been the purification of the target of interest. As we move into a new era in medicine, therapies should aim to fix the clinical problem at hand while leaving the rest of a patient's body unperturbed. In the case of a tissue or organ system, the identification of the stem cell can allow for either targeted removal or repopulation of that tissue. The former is best exemplified by the identification of cancer stem cells (CSCs), which represent a rare, self-renewing population of malignant cells that have the capacity to differentiate and recapitulate the entire makeup of a bulk tumor. Targeted therapies to CSCs have long been desired as non-specific therapies that work by attacking rapidly dividing cells may cull a large mass but if they leave quiescent CSCs untouched, there may be a chance of recurrence. The following body of work will dive further into this topic and describe a study that yielded a putative CSC population in pancreatic neuroendocrine tumors, as well as potential targeted therapies. The latter scenario of tissue repopulation is best exemplified by bone marrow transplantation, where one's entire hematopoietic system can be replaced by donor blood forming stem cells. This technique is utilized for curing hematopoietic malignancies and genetic disorders; however, its use is limited by side effects related to the non-specific drugs used to prepare a patient for transplant and the impure donor cell grafts that are infused. This dissertation will also discuss work that outlines a novel approach for specifically ablating and engrafting hematopoietic stem cells (HSCs) that was made possible by the purification and subsequent characterization of this population by others


Type of resource text
Form electronic resource; remote; computer; online resource
Extent 1 online resource
Place California
Place [Stanford, California]
Publisher [Stanford University]
Copyright date 2020; ©2020
Publication date 2020; 2020
Issuance monographic
Language English


Author George, Benson M
Degree supervisor Weissman, Irving L
Thesis advisor Weissman, Irving L
Thesis advisor Mackall, Crystal
Thesis advisor Majeti, Ravindra, 1972-
Thesis advisor Roncarolo, Maria-Grazia
Thesis advisor Shizuru, Judith Anne
Degree committee member Mackall, Crystal
Degree committee member Majeti, Ravindra, 1972-
Degree committee member Roncarolo, Maria-Grazia
Degree committee member Shizuru, Judith Anne
Associated with Stanford University, Cancer Biology Program.


Genre Theses
Genre Text

Bibliographic information

Statement of responsibility Benson M. George
Note Submitted to the Cancer Biology Program
Thesis Thesis Ph.D. Stanford University 2020
Location electronic resource

Access conditions

© 2020 by Benson M George
This work is licensed under a Creative Commons Attribution 3.0 Unported license (CC BY).

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