High bandwith AFM imaging in fluid using interdigitated probes
Abstract/Contents
- Abstract
- High speed imaging of local material properties of soft materials such as biological cells using the Atomic Force Microscope (AFM) is an area of active research. An understanding of nanomechanical properties and local adhesive forces on cell surfaces would greatly improve our understanding of fundamental cellular processes. The interaction force between the probe tip and the sample surface contains rich information about the properties of the sample. However, due to the limited bandwidth of traditional AFM cantilevers, a significant portion of this rich information is lost. Interdigitated AFM probes overcome this limitation by incorporating a high bandwidth diffraction grating based force sensor on the cantilever to measure the time-varying interaction forces with adequately high bandwidth while operating in AC mode AFM. In this work, a new design for interdigitated AFM probes is presented. The new probes have been optically redesigned such that they can be easily operated in both air as well as liquid environments by making small adjustments to manner in which they are used. The mechanical design of the probes allows for simpler fabrication processes which brings them closer to standard probe manufacturing. A new analytical model is presented which allows greater insight into the mechanical properties of the probes. Furthermore, the system design, characterization and signal processing that go along with the use of these probes are discussed. The ability of these probes to map local material properties with high resolution is demonstrated by imaging a wide range of samples in fluid.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2013 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Vijayraghavan, Karthik |
|---|---|
| Associated with | Stanford University, Department of Electrical Engineering. |
| Primary advisor | Solgaard, Olav |
| Thesis advisor | Solgaard, Olav |
| Thesis advisor | Khuri-Yakub, Butrus T, 1948- |
| Thesis advisor | Melosh, Nicholas A |
| Advisor | Khuri-Yakub, Butrus T, 1948- |
| Advisor | Melosh, Nicholas A |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Karthik Vijayraghavan. |
|---|---|
| Note | Submitted to the Department of Electrical Engineering. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2013. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2013 by Karthik Vijayraghavan
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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