Structural and biochemical analysis of phosphorylation events during Wnt signal transduction
Abstract/Contents
- Abstract
- A great deal is known about elements of the Wnt signal transduction cascade and the [beta]-catenin destruction complex, but a complete molecular understanding of their function has remained elusive. Many of the proteins that are necessary for [beta]-catenin destruction, including GSK-3, CK1, Axin, and APC, also have roles in stabilizing [beta]-catenin on Wnt stimulation, thus confounding genetic analyses and cell culture overexpression studies in difficult-to-predict ways. In addition, Wnt signal transduction proteins such as GSK-3, CK1, and APC play critical roles in other signal transduction and metabolic cascades within cells, including such diverse pathways as glycogen regulation, circadian rhythm, spindle formation during mitosis, and microtubule stability. In order to carefully dissect Wnt signal transduction in the absence of these confounding factors, biochemical and structural studies were carried out on proteins from the destruction complex and the Wnt signaling cascade, with emphasis placed on the role of phosphorylation events on the APC, [beta]-catenin, and LRP5/6 proteins during the transition from [beta]-catenin destruction to activation.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2013 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Stamos, Jennifer Lynn |
|---|---|
| Associated with | Stanford University, Department of Molecular and Cellular Physiology. |
| Primary advisor | Ferrell, James Ellsworth |
| Primary advisor | Weis, William I |
| Thesis advisor | Ferrell, James Ellsworth |
| Thesis advisor | Weis, William I |
| Thesis advisor | Jardetzky, Theodore |
| Thesis advisor | Nusse, Roel, 1950- |
| Advisor | Jardetzky, Theodore |
| Advisor | Nusse, Roel, 1950- |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Jennifer Lynn Stamos. |
|---|---|
| Note | Submitted to the Department of Molecular and Cellular Physiology. |
| Thesis | Ph.D. Stanford University 2013 |
| Location | electronic resource |
Access conditions
- Copyright
- © 2013 by Jennifer Lynn Stamos
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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