16S rRNA data and metadata for "Chemoproteomic identification of a DPP4 homolog in Bacteroides thetaiotaomicron"
Abstract/Contents
- Abstract
- Serine hydrolases play important roles in signaling and human metabolism, yet little is known about their functions in gut commensal bacteria. Using bioinformatics and chemoproteomics, we identify serine hydrolases in the gut commensal Bacteroides thetaiotaomicron that are specific to the Bacteroidetes phylum. Two are predicted homologs of the human protease dipeptidyl peptidase 4 (hDPP4), a key enzyme that regulates insulin signaling. Functional studies reveal that BT4193 is a true homolog of hDPP4 that can be inhibited by FDA-approved type 2 diabetes medications targeting hDPP4, while the other is a misannotated proline-specific triaminopeptidase. We demonstrate that BT4193 is important for envelope integrity and that loss of BT4193 reduces B. thetaiotaomicron fitness during in vitro growth within a diverse community. However, neither function is dependent on BT4193 proteolytic activity, suggesting a scaffolding or signaling function for this bacterial protease.
Description
| Type of resource | Dataset, text |
|---|---|
| Date created | [ca. April 2022] |
| Publication date | April 16, 2023; April 15, 2023 |
Creators/Contributors
| Author | Keller, Laura J. | |
|---|---|---|
| Author | Nguyen, Taylor H. |
https://orcid.org/0000-0002-1288-8882
(unverified)
|
| Author | Liu, Lawrence | |
| Author | Hurysz, Brianna M. | |
| Author | Lakemeyer, Markus | |
| Author | Guerra, Matteo | |
| Author | Gelsinger, Danielle J. | |
| Author | Chanin, Rachael | |
| Author | Ngo, Nhi | |
| Author | Lum, Kenneth M. | |
| Author | Faucher, Franco | |
| Author | Ipock, Phillip | |
| Author | Niphakis, Micah J. | |
| Author | Bhatt, Ami S. | |
| Author | O'Donoghue, Anthony J. | |
| Author | Huang, Kerwyn Casey | |
| Author | Bogyo, Matthew |
Subjects
| Subject | activity-based protein profiling; vancomycin; polymyxin B; gut bacterial communities; synthetic communities; microbiota; microbiome |
|---|---|
| Genre | Data |
| Genre | Manuscript |
| Genre | Data sets |
| Genre | Dataset |
Bibliographic information
| Related item | |
|---|---|
| DOI | https://doi.org/10.25740/rn970zy4428 |
| Location | https://purl.stanford.edu/rn970zy4428 |
Access conditions
- Use and reproduction
- User agrees that, where applicable, content will not be used to identify or to otherwise infringe the privacy or confidentiality rights of individuals. Content distributed via the Stanford Digital Repository may be subject to additional license and use restrictions applied by the depositor.
- License
- This work is licensed under an Open Data Commons Attribution License v1.0.
Preferred citation
- Preferred citation
- Keller, L., Nguyen, T., Liu, L., Hurysz, B., Lakemeyer, M., Guerra, M., Gelsinger, D., Chanin, R., Ngo, N., Lum, K., Faucher, F., Ipock, P., Niphakis, M., Bhatt, A., O'Donoghue, A., Huang, K., and Bogyo, M. (2023). 16S rRNA data and metadata for "Chemoproteomic identification of a DPP4 homolog in Bacteroides thetaiotaomicron". Stanford Digital Repository. Available at https://purl.stanford.edu/rn970zy4428. https://doi.org/10.25740/rn970zy4428.
Collection
Stanford Research Data
View other items in this collection in SearchWorksContact information
Also listed in
Loading usage metrics...