Label-free optical detection of bioelectric potentials using electrochromic thin films
Abstract/Contents
- Abstract
- Recording electrical signals from cells with electrode arrays is limited by the number of addressable cells, fixed recording sites, and incompatibility with optical tools. Herein, we overcome these limitations by recording field potentials using electrochromism to translate electrical signals into changes in optical absorbance. We use a prism-coupled attenuated total internal reflection laser setup to interrogate the small changes in color by examining the light intensity of a laser beam reflected by the film. We apply this label-free technique to genetically modified cells, human iPSC-derived cardiomyocytes, primary mouse hippocampal neurons, dorsal root ganglion neurons and organotypic hippocampal brain slices. This platform is a powerful method to study electrically active cells which are difficult to transfect since the field of vision can be redirected by stirring the laser onto to the desired region of interest.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2019; ©2019 |
| Publication date | 2019; 2019 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Alfonso, Felix Steven |
|---|---|
| Degree supervisor | Cui, Bianxiao |
| Thesis advisor | Cui, Bianxiao |
| Thesis advisor | Chidsey, Christopher E. D. (Christopher Elisha Dunn) |
| Thesis advisor | Martinez, Todd J. (Todd Joseph), 1968- |
| Degree committee member | Chidsey, Christopher E. D. (Christopher Elisha Dunn) |
| Degree committee member | Martinez, Todd J. (Todd Joseph), 1968- |
| Associated with | Stanford University, Department of Chemistry. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Felix S. Alfonso. |
|---|---|
| Note | Submitted to the Department of Chemistry. |
| Thesis | Thesis Ph.D. Stanford University 2019. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2019 by Felix Steven Alfonso
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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