NeuroPlant
Abstract/Contents
- Abstract
- We developed an efficient screening platform to evaluate chemotactic behavioral responses (attraction/repulsion) in C. elegans when exposed to plant derived small molecules. This platform uses multiwell plates, standard liquid handling equipment, flatbed scanners, a custom data management workflow, and computer-vision based software for data analysis. With this system, we screened 96 conditions (90 test and 6 reference compounds) in triplicate against the N2 wild-type C. elegans strain in 3 experimental days. We identified 37 SMs that evoke detectable responses in chemotaxis assays and advanced these 37 compounds into screens using tax-4, osm-9, and tax-4;osm-9 mutants. Taken together these initial results move us closer to our goal of better understanding the molecular mechanisms of chemosensory signal transduction and the identification of SM/receptor pairs. Overall, this repository contains the raw image files (.tiff), metadata and worm location files (.csv) for all analyzed images associated with the screen described above.
Description
| Type of resource | still image, Dataset |
|---|---|
| Date created | [ca. August 12, 2021 - April 2022] |
| Publication date | May 6, 2024 |
Creators/Contributors
https://orcid.org/0000-0003-4152-3652
Subjects
| Subject | Neurosciences |
|---|---|
| Subject | Caenorhabditis elegans |
| Subject | Chemotaxis |
| Subject | Methods |
| Subject | High-throuput |
| Subject | Small molecule |
| Subject | Natural products |
| Subject | plant-based compounds |
| Subject | Nematodes |
| Subject | phenotyping platform |
| Genre | Image |
| Genre | Data |
| Genre | Data sets |
| Genre | Dataset |
Bibliographic information
| Related item | |
|---|---|
| DOI | https://doi.org/10.25740/rh109rs1058, https://doi.org/10.25740/rh109rs1058 |
| Location | https://purl.stanford.edu/rh109rs1058 |
Access conditions
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 4.0 International license (CC BY-NC).
Preferred citation
- Preferred citation
- Fryer, E., Guha, S., Rogel-Hernandez, L., Logan-Garbisch, T., Farah, H., Rezaei, E., Mollhoff, I., Nekimken, A., Xu, A., Seyahi, L., Fechner, S., Druckmann, S., Clandinin, T., Rhee, S., and Goodman, M. (2024). NeuroPlant -. Stanford Digital Repository. Available at https://purl.stanford.edu/rh109rs1058. https://doi.org/10.25740/rh109rs1058.
Collection
Stanford Research Data
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